Anti-macroH2A.1 Rabbit Polyclonal Antibody for WB, ELISA - O75367

Core Product Specifications and Parameters

Biological Background: macroH2A.1 Function and Localization

• macroH2A.1 is a core histone variant encoded by the MACROH2A1 gene, which produces multiple isoforms through alternative splicing, including isoform 1 (which binds poly-ADP-ribose) and isoform 2 (which does not).
• Replaces conventional H2A in a subset of nucleosomes and functions as a transcriptional repressor. Related references: PMID:12718888, PMID:15621527, PMID:16428466
• Nucleosomes compact DNA into chromatin, regulating transcription, DNA repair, replication, and chromosomal stability. Related references: PMID:15897469
• Essential for stable X chromosome inactivation. Related references: PMID:15897469
• Inhibits transcription factor binding (e.g., NF-kappa-B) and disrupts SWI/SNF chromatin remodeling. Related references: PMID:12718888, PMID:16428466
• Suppresses histone acetylation by EP300 and recruits class I HDACs, leading to a hypoacetylated chromatin state. Related references: PMID:16107708, PMID:16428466
• Isoform 1 binds poly-ADP-ribose and O-acetyl-ADP-ribose, playing a key role in NAD+ metabolism by capping PAR chains and limiting NAD+ consumption. Related references: PMID:15902274, PMID:23022728
• Predominantly nuclear, localizing to chromosomes; widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 200–300; thus, the antibody is expected to recognize linear epitopes and may be suitable for denaturing conditions in WB.
• In WB, a band at approximately 39 kDa is expected; consider including appropriate loading controls and validating in both human and rat lysates due to reported cross-reactivity.
• For ELISA, the antibody may be useful for detecting macroH2A.1 in purified nucleosome preparations or recombinant proteins; optimize coating and detection conditions as needed.
• Since macroH2A.1 is a chromatin-bound protein, consider chromatin extraction or nuclear fractionation protocols to enrich the target protein before detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for chromatin biology and epigenetic targets. This macroH2A.1 polyclonal antibody is part of our extensive portfolio for histone variant research, and our team can assist with rare target raw-material sourcing and technical consultation to support your assay development from concept to clinic.