Anti-macroH2A.1 Polyclonal Antibody for WB, IF/ICC, ELISA - O75367

Core Product Specifications and Parameters

Biological Background: macroH2A.1 Function and Localization

• Core histone macro-H2A.1 is a variant histone H2A that replaces conventional H2A in a subset of nucleosomes, where it represses transcription. Nucleosomes compact DNA and regulate accessibility for processes like DNA repair and replication. Related references: PMID:12718888, PMID:15621527, PMID:16428466, PMID:15897469
• Involved in stable X chromosome inactivation, inhibiting binding of transcription factors including NF-kappa-B and interfering with SWI/SNF remodeling complexes. Related references: PMID:12718888, PMID:16428466
• Inhibits histone acetylation by EP300 and recruits class I HDACs, leading to a hypoacetylated chromatin state. Related references: PMID:16107708, PMID:16428466
• An isoform specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose, playing a key role in NAD+ metabolism by capping PAR chains and limiting NAD+ consumption, thus promoting mitochondrial respiration. Related references: PMID:15902274, PMID:23022728
• Another isoform does not bind poly-ADP-ribose and instead represses SOD3 gene expression. Related references: PMID:15902274, PMID:23022728
• Localizes to the nucleus and chromosomes.
• Subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitination, which regulate its functions.
• Widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 123–372 of human macroH2A.1, including regions common to both main isoforms; the polyclonal antibody may recognize multiple splice variants. Validate species cross-reactivity in your sample system as sequences may vary.
• For Western blot (WB), expect a band around 39 kDa; post-translational modifications or isoform differences may result in multiple or shifted bands. Optimize blocking and antibody dilution for your specific lysate.
• In immunofluorescence (IF/ICC), a predominantly nuclear staining pattern is anticipated. Use appropriate fixation and permeabilization protocols to preserve nuclear epitopes.
• For ELISA, consider pairing with a matched detection antibody if developing a sandwich assay; the polyclonal nature may offer high sensitivity but requires careful titration.
• The antibody has been validated by the supplier for WB, IF/ICC, and ELISA with human, mouse, and rat samples; researchers should perform independent validation for their specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated IVD raw materials, including polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. This macroH2A.1 antibody supports research into epigenetic regulation, chromatin biology, and NAD+ metabolism. We streamline procurement from multiple manufacturers, ensuring consistent supply and technical support from concept to clinic.