Anti-M6PR Polyclonal Antibody for WB, IHC-P, ELISA - P20645

Core Product Specifications and Parameters

Biological Background: M6PR Function and Localization

• The M6PR gene encodes the cation-dependent mannose-6-phosphate receptor (46 kDa mannose 6-phosphate receptor, MPR 46), a type I transmembrane receptor.
• This receptor mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes, binding mannose-6-phosphate residues on the enzymes.
• The receptor-ligand complex is internalized and delivered to an acidic pre-lysosomal compartment, where the low pH triggers dissociation of the cargo.
• Subcellularly, M6PR is predominantly localized to the lysosome membrane.
• Key structural features include a signal peptide, a single transmembrane helix, and multiple post-translational modifications such as glycosylation and disulfide bond formation.
• The protein is also phosphorylated and has a predicted molecular weight of approximately 31 kDa, consistent with the specification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of M6PR (aa 27-185), suggesting the antibody likely recognizes the mature receptor after signal peptide cleavage.
• For Western blotting, a band near 31 kDa is expected; consider using reducing conditions and appropriate positive controls.
• IHC-P can be performed on formalin-fixed paraffin-embedded tissues; validate with isotype controls and, if possible, peptide blocking.
• ELISA may be carried out using the recombinant immunogen as a coating antigen; titrate the antibody to determine optimal working concentration.
• Given cross-reactivity with mouse and rat, this antibody is suitable for comparative studies across species; however, always confirm specificity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. The Anti-M6PR polyclonal antibody is one component in our portfolio of validated tools for lysosomal storage disorder research and assay development. Beyond antibodies, we offer bulk ancillary reagents and access to rare-target raw materials, streamlining your supply chain with a single, reliable source.