Anti-LZTR1 Rabbit Polyclonal Antibody for WB, ELISA - Q8N653

Core Product Specifications and Parameters

Biological Background: LZTR1 Function and Localization

• LZTR1 (Leucine-zipper-like transcriptional regulator 1), also known as BTBD29, functions as a substrate-specific adapter for the BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, despite its name suggesting a transcriptional role. Related references: PMID:30442762 PMID:30442766 PMID:30481304
• It mediates the ubiquitination of Ras GTPases (M-Ras/MRAS, K-Ras/KRAS, N-Ras/NRAS, H-Ras/HRAS) and the Ras-like protein RIT1, targeting them for proteasomal degradation. Related references: PMID:30442762 PMID:30442766 PMID:30481304
• LZTR1 acts as a negative regulator of the RAS-MAPK signaling pathway by controlling Ras protein levels and reducing their association with membranes. Related references: PMID:30442762 PMID:30442766 PMID:30481304
• The protein localizes to the endomembrane system, including recycling endosomes and the Golgi apparatus, consistent with its role in membrane-associated Ras regulation.
• LZTR1 contains Kelch repeats and a BTB domain, characteristic of BTB-Kelch proteins involved in ubiquitin ligase complexes.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity. Mutations in LZTR1 are associated with Noonan syndrome and other RASopathies, underscoring its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen spans the central region (aa 300-545) of LZTR1, enabling detection of both full-length and potential isoforms or cleavage products.
• For Western blot, denaturing conditions with positive controls such as HeLa or HEK293 lysates are suggested; confirm band specificity using siRNA knockdown or knockout models.
• ELISA may require direct coating of recombinant LZTR1 protein or peptide; optimize antibody dilution and blocking conditions to minimize background.
• LZTR1’s membrane association implies that subcellular fractionation or enrichment of endosomal/Golgi components may improve signal in some sample types.
• Avoid excessive stripping between detections, as LZTR1 may be sensitive to harsh conditions; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers with a comprehensive range of raw materials for assay development and production. This anti-LZTR1 antibody is part of our portfolio for ubiquitin-proteasome pathway and RAS-MAPK signaling research, offering consistent performance for WB and ELISA workflows. Beyond antibodies, we provide validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and rare target sourcing to seamlessly bridge your project from concept to clinic.