Anti-LYVE1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q8BHC0

Core Product Specifications and Parameters

Biological Background: LYVE1 Function and Localization

• LYVE1 is a ligand-specific transporter that mediates trafficking between the trans-Golgi network and the plasma membrane, and plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). Related references: PMID:10187853
• It functions as a hyaluronan (HA) transporter, either mediating HA uptake for catabolism within lymphatic endothelial cells or transport into the lumen of afferent lymphatic vessels.
• LYVE1 binds to pericellular hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium.
• Also known as cell surface retention sequence-binding protein 1 (CRSBP-1) and extracellular link domain-containing protein 1 (XLKD1), reflecting its alternative functions.
• Localized to the membrane, consistent with its roles in transport and cell adhesion.
• Post-translational modifications include glycosylation and disulfide bond formation, and it is a glycoprotein receptor with a signal peptide and a transmembrane helix.
• Mouse LYVE1 has a molecular weight of approximately 34.6kDa (34574 Da).

Experimental Guidance and Technical Tips

• For Western blotting (WB), consider a starting dilution range of 1:500–1:2000 and use protein markers to confirm the ~34kDa band.
• In immunohistochemistry-paraffin (IHC-P) and immunofluorescence-paraffin (IF-P), validate the antibody in lymphatic-rich tissues such as lymph nodes or skin from mouse and rat.
• The immunogen corresponds to the extracellular region (amino acids 30–228), suggesting potential recognition of both native and denatured LYVE1.
• Cross-reactivity with rat is indicated; verify performance in rat samples prior to experimental use.
• For ELISA, optimize coating and detection conditions according to standard protocols for monoclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including this high-quality LYVE1 monoclonal antibody for lymphatic biology and tumor metastasis studies. Our supply capabilities extend to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, ensuring a streamlined path from research to clinical application. With expertise from concept to clinic, CamelBio is your trusted partner for all IVD raw-material needs.