Anti-LYVE1 Rabbit Monoclonal Antibody for WB, ELISA - Q9Y5Y7

Core Product Specifications and Parameters

Biological Background: LYVE1 Function and Localization

• Lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE1) is a transmembrane receptor primarily expressed on lymphatic endothelial cells.
• It functions as a ligand-specific transporter, mediating trafficking between the trans-Golgi network (TGN) and the plasma membrane, and plays a role in autocrine regulation of cell growth via cell surface retention sequence binding (CRS).
• LYVE1 may act as a hyaluronan (HA) transporter, either facilitating HA uptake for catabolism in lymphatic endothelial cells or its transport into afferent lymphatic vessels for degradation in lymph nodes. (PubMed:10037799) Related references: PMID:10037799
• The receptor binds to pericellular hyaluronan matrices on leukocytes, promoting cell adhesion and migration through the lymphatic endothelium. (PubMed:26823460) Related references: PMID:26823460
• It belongs to the Link domain-containing protein family, also designated as CRSBP-1 or extracellular link domain-containing protein 1.
• Subcellularly, LYVE1 is localized to the cell membrane.
• Key structural features include a signal peptide, a transmembrane helix, disulfide bonds, and glycosylation sites, classifying it as a glycoprotein receptor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100), suggesting the antibody likely recognizes an epitope in the extracellular domain. For western blot, the predicted molecular weight is approximately 35 kDa; however, glycosylation may result in a higher observed band. Validate in relevant lymphatic endothelial cell lines or tissues.
• This rabbit monoclonal antibody is suitable for ELISA applications; consider using the immunogen peptide as a positive control to assess coating and detection.
• Cross-reactivity with mouse and rat LYVE1 has been reported, making this antibody a potential tool for comparative studies across species; however, verify reactivity in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to validated IVD raw materials, including monoclonal and polyclonal antibodies, antigen pairs, and ancillary reagents. For lymphatic biology and hyaluronan receptor research, we offer bulk sourcing options and technical consultation to support your assay development from early discovery to clinical application.