Anti-LysRS/KARS Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q15046

Core Product Specifications and Parameters

Biological Background: LysRS/KARS Function and Localization

• Catalyzes the specific attachment of lysine to its cognate tRNA in a two-step reaction: amino acid activation by ATP to form AA-AMP and transfer to the acceptor end of tRNA. Related references: PMID:18029264, PMID:18272479, PMID:9278442
• When secreted, acts as a signaling molecule that induces immune response through activation of monocyte/macrophages. Related references: PMID:15851690
• Catalyzes synthesis of diadenosine tetraphosphate (Ap4A), mediating disruption of HINT1-MITF complex and activation of MITF transcriptional activity. Related references: PMID:14975237, PMID:19524539, PMID:23159739
• Interacts with HIV-1 GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
• Subcellular localization includes cytoplasm (cytosol), nucleus, cell membrane, secreted, and mitochondrion.
• Keywords: Aminoacyl-tRNA synthetase, ATP-binding, Ligase, Transferase, Protein biosynthesis, Host-virus interaction, Disease variant (Charcot-Marie-Tooth disease, deafness, intellectual disability), Phosphoprotein, Acetylation, Alternative splicing, Membrane, Mitochondrion, Nucleus, Secreted, Reference proteome.
• Molecular weight: 68,048 Da (UniProt).

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at ~68 kDa. Validate with positive control lysates from human, mouse, or rat sources as per cross-reactivity data.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider suitable antigen retrieval methods and appropriate fixation conditions.
• For immunoprecipitation (IP), confirm antibody compatibility with your lysis buffer and validate the binding efficiency.
• For ELISA, titrate the antibody and validate against recombinant or purified target protein.
• The immunogen corresponds to amino acids 500-597 (C-terminal region), away from the catalytic domain. If functional blocking is required, validate that the antibody does not inhibit enzymatic activity.
• Due to multiple subcellular localizations, consider cell fractionation or appropriate controls to confirm localization in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio streamlines your diagnostic development with validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For LysRS/KARS research, we provide reliable sourcing for critical raw materials targeting aminoacyl-tRNA synthetase pathways, immune signaling, and host-virus interaction studies. Our supply capabilities extend to rare target raw materials, ensuring you get the components you need from concept to clinic.