Anti-LysRS/KARS Polyclonal Antibody for WB, IF/ICC, ELISA - Q15046

Core Product Specifications and Parameters

Biological Background: LysRS/KARS Function and Localization

• Catalyzes the two-step attachment of lysine to tRNA(Lys): activation of lysine by ATP to form Lys-AMP, followed by transfer to the 3'-end of tRNA(Lys), a fundamental step in cytoplasmic protein synthesis. Related references: PMID:18029264, PMID:18272479, PMID:9278442
• Upon secretion, acts as a signaling molecule that triggers immune responses by activating monocytes/macrophages (PubMed:15851690). Related references: PMID:15851690
• Catalyzes the synthesis of the dinucleotide diadenosine tetraphosphate (Ap4A), which disrupts the HINT1-MITF complex, thereby activating MITF transcriptional activity (PubMed:14975237, PubMed:19524539, PubMed:23159739). Related references: PMID:14975237, PMID:19524539, PMID:23159739
• During HIV-1 infection, interacts with the viral GAG protein to facilitate selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
• Found in the cytoplasm, cytosol, nucleus, cell membrane, secreted, and mitochondria; its multifunctional roles are reflected in this broad subcellular distribution.
• Mutations in KARS1 are associated with Charcot-Marie-Tooth disease, deafness (DFNB89), and intellectual disability, highlighting its neurological importance.
• Undergoes acetylation and phosphorylation, which may regulate its enzymatic and non-canonical functions.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant protein spanning amino acids 298-597 of human LysRS. This region covers the C-terminal catalytic domain; thus, the antibody may recognize full-length and certain truncated isoforms if the epitope is preserved.
• For WB, a band around 68 kDa is expected, consistent with the calculated molecular weight. Consider optimizing blocking and detection conditions for the sample type and species.
• In IF/ICC, appropriate fixation and permeabilization methods should be used to access different subcellular pools (cytoplasmic, nuclear, mitochondrial).
• For ELISA, the antibody can be used in combination with appropriate capture/detection reagents; titration is recommended for each assay format.
• Cross-reactivity has been reported with human, mouse, and rat samples, but sequence homology suggests potential reactivity in other species; validate in the relevant experimental system.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare target raw materials. For targets like LysRS/KARS, relevant to protein synthesis and neurological disease research, we offer comprehensive supply solutions to accelerate your assay development and research applications.