Anti-LY86 Polyclonal Antibody for WB, IHC-P, ELISA - O95711

Core Product Specifications and Parameters

Biological Background: LY86 Function and Localization

• LY86 (Lymphocyte antigen 86), also known as MD-1, is a secreted glycoprotein that functions as a key component of the innate immune system.
• It cooperates with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production.
• LY86 is important for the efficient cell surface expression of CD180, a receptor involved in B-cell activation.
• The protein is localized to the extracellular space, consistent with its role as a secreted mediator.
• It is highly expressed in B-cells, monocytes, and tonsil, aligning with its role in immune surveillance and response.
• Post-translational modifications include disulfide bond formation and glycosylation, as revealed by direct protein sequencing and 3D structure analysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 21-162), which is predicted to be exposed on the native secreted protein; consider this when designing detection strategies for native versus denatured forms.
• For Western blotting, the predicted molecular weight is 18 kDa; however, glycosylation may shift the observed band upward, so deglycosylation enzymes may be worth considering to confirm identity.
• When performing IHC-P on human tissues, optimal results are often achieved with antigen retrieval methods; validation in B-cell-rich areas such as tonsil is recommended.
• For ELISA, given the secreted nature of LY86, detection in cell culture supernatants or body fluids is feasible; cross-reactivity with mouse LY86 allows for translational studies in mouse models.
• Always validate the antibody in the relevant sample system with appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop channel for IVD raw materials, supporting diagnostic manufacturers and research institutes from concept to clinic. For projects targeting innate immunity and LPS signaling, we offer this anti-LY86 polyclonal antibody alongside a full portfolio of validated reagents—from optimized monoclonal/polyclonal antibodies and bulk ancillary reagents to custom rare-target sourcing. Let us streamline your procurement and help bridge the gap between research and reliable diagnostics.