Anti-LY75 (DEC-205/CD205) Rabbit Polyclonal Antibody for WB/ELISA - O60449

Core Product Specifications and Parameters

Biological Background: Lymphocyte Antigen 75 Function and Localization

• Lymphocyte antigen 75 (LY75), also known as DEC-205, CD205, gp200-MR6, and CLEC13B, is a type I transmembrane protein belonging to the C-type lectin domain family 13. It contains multiple C-type lectin-like domains (CTLDs) and is expressed as several isoforms due to alternative splicing, with isoform 2 and 3 detected in Hodgkin and Reed-Sternberg (HRS) cells.
• LY75 functions as an endocytic receptor that efficiently captures and internalizes antigens from the extracellular space, directing them to specialized antigen-processing compartments within antigen-presenting cells. This role is critical for cross-presentation and initiation of immune responses.
• In B-lymphocytes, LY75 engagement causes reduced proliferation, suggesting an inhibitory or regulatory role in B-cell homeostasis.
• LY75 is prominently expressed in the spleen, thymus, colon, and peripheral blood lymphocytes. At the cellular level, it is detected in myeloid and B-lymphoid cell lines, consistent with its role in professional antigen-presenting cells.
• Subcellularly, LY75 is a membrane protein containing a signal peptide, a single transmembrane helix, and a cytoplasmic tail. It is classified under endocytosis, receptor, and glycoprotein keywords, and undergoes phosphorylation and disulfide bond formation.
• The molecular weight of the canonical isoform is approximately 198 kDa, consistent with extensive glycosylation and domain structure.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant immunogen spanning amino acids 216–501, which includes multiple CTLDs, and may recognize linear and conformational epitopes in both human and mouse LY75.
• For Western blot (WB), the predicted molecular weight is ~198 kDa. Use appropriate gel percentages (e.g., 6–8% SDS-PAGE) and membrane types (PVDF) to ensure efficient transfer of this high-molecular-weight protein.
• The antibody is supplied for WB and ELISA applications. For WB, consider testing a range of dilutions on lysates from LY75-expressing tissues or cell lines (e.g., spleen, thymus, Raji, or Hodgkin lymphoma lines). Include positive and negative controls to verify specificity.
• Given the polyclonal nature, batch-to-batch consistency should be assessed if used in quantitative assays. Validate cross-reactivity in the mouse system using appropriate tissue or cell controls before designing comparative experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials. This LY75 antibody supports R&D in immune function, antigen presentation, and lymphoma research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—helping you move from concept to clinic with efficiency.