Anti-Ly-6G Monoclonal Antibody for FC - P35461

Core Product Specifications and Parameters

Biological Background: Ly-6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also known as Ly-6G.1, is a mouse cell surface protein encoded by the Ly6g gene.
• Ly-6G is a GPI-anchored glycoprotein localized to the cell membrane, as indicated by the presence of a GPI-anchor and signal peptide.
• It contains a disulfide bond typical of the Ly-6/uPAR superfamily, contributing to structural stability.
• The protein is a lipoprotein, likely involved in membrane anchoring and signaling.
• Tissue specificity data from UniProt indicate Ly-6G expression in bone marrow.
• Keywords such as “Glycoprotein” support post-translational modifications; the mature protein has a predicted molecular weight of ~14.3 kDa, but glycosylation results in an observed band around 28 kDa.

Experimental Guidance and Technical Tips

• As a rabbit monoclonal antibody, use a rabbit IgG isotype control for flow cytometry experiments to assess non-specific binding.
• Titrate the antibody to determine the optimal concentration for your specific flow cytometry panel and cell population.
• Since Ly-6G is expressed on the cell surface, standard surface staining protocols without permeabilization are typically sufficient; however, validate in your experimental system.
• Validate antibody performance in positive and negative control samples, such as bone marrow cells from wild-type and Ly-6G knockout mice.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and researchers, providing one-stop access to IVD raw materials. For immunology research requiring myeloid cell markers such as Ly-6G, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our supply network also supports rare target raw-material sourcing to accelerate your assay development.