Anti-LXRα Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13133

Core Product Specifications and Parameters

Biological Background: LXRα Function and Localization

• LXRα (oxysterols receptor LXR-alpha, encoded by NR1H3) is a nuclear receptor that functions as a ligand-activated transcription factor (PubMed:19481530, PubMed:25661920, PubMed:37478846). Related references:
  PMID:19481530, PMID:25661920, PMID:37478846
• Interaction with RXR shifts the heterodimer into an active ligand-binding complex that mediates retinoid signaling through LXRES elements (PubMed:37478846). Related references:
  PMID:37478846
• Key regulator of cholesterol homeostasis; drives MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8 to promote cholesterol uptake (PubMed:19481530). Related references:
  PMID:19481530
• Induces LPCAT3-dependent ER phospholipid remodeling in hepatocytes, which in turn activates SREBF1 processing and lipogenesis, and facilitates VLDL maturation.
• Incorporates arachidonate into ER membrane phosphatidylcholines, increasing membrane dynamics and counteracting lipid-induced ER stress and inflammation.
• Predominantly expressed in visceral organs—liver, kidney, intestine, and to a lesser extent spleen and adrenals—and shuttles between nucleus and cytoplasm.
• Contains zinc-finger motifs, undergoes alternative splicing and ubiquitination, highlighting potential isoform diversity and post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human LXRα; alternative splicing or N-terminal modifications in certain cell types may influence epitope recognition.
• For Western blotting, a band near 50 kDa is expected; reducing conditions and appropriate ladder are recommended for size confirmation.
• In immunohistochemistry (paraffin sections), antigen retrieval may be necessary and should be optimized for the tissue type, especially for liver or kidney.
• For IF/ICC, co-localization with nuclear and/or cytoplasmic markers can help verify LXRα distribution under different metabolic conditions.
• When using the antibody in rodent samples, confirm cross-reactivity by sequence alignment and pilot experiments in mouse or rat lysates.
• ELISA application may be suitable for detecting recombinant LXRα or in sandwich pair formats; validate assay conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing bridge, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support every stage of diagnostic development. For nuclear receptor biology and cholesterol metabolism targets like LXRα, our supply network and technical consultation streamline assay development from concept to clinic.