Anti-LXR beta/NER Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P55055

Core Product Specifications and Parameters

Biological Background: LXR beta/NER Function and Localization

• Also known as Liver X receptor beta, Nuclear receptor NER, NR1H2, and RIP15 (Gene ID: 7376). Encodes a ~51 kDa nuclear receptor protein.
• Functions as a ligand-dependent transcriptional activator, binding to DNA elements with the consensus sequence 5'-AGGTCA-3' (DR-4) (PubMed:25661920). Related references: PMID:25661920
• Regulates cholesterol homeostasis by mediating MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8, thereby controlling lipoprotein uptake.
• Interplays with RORA to regulate liver metabolic genes and induces LPCAT3-dependent phospholipid remodeling in ER membranes, driving lipogenesis and VLDL assembly.
• Acts as an anti-inflammatory corepressor during the hepatic acute phase response by preventing N-Cor corepressor complex dissociation (PubMed:20159957). Related references: PMID:20159957
• Localizes to the nucleus and is ubiquitously expressed across tissues.
• Belongs to the nuclear receptor superfamily, featuring zinc-finger DNA-binding domains and ligand-binding domains; undergoes Ubl conjugation and isopeptide bond formation.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide spanning amino acids 180-260 of human LXR beta/NER. For WB, consider using whole cell lysates or nuclear extracts.
• A band near 51 kDa is expected. Optimize blocking and antibody dilution empirically using lysates from human, mouse, or rat tissues known to express LXR beta (e.g., liver, kidney).
• For IF/ICC, fixation and permeabilization conditions are critical for nuclear protein detection. Use appropriate controls and validate signal specificity.
• In ELISA, the antibody can serve as a capture or detection reagent; pairing with a matched monoclonal antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting research into nuclear receptor signaling and cholesterol metabolism. Whether you need ready-to-use LXR beta/NER antibodies or custom sourcing for rare targets, our integrated supply chain bridges discovery to diagnostic development.