Anti-LXR beta/NER Rabbit Polyclonal Antibody for WB, ELISA - P55055

Core Product Specifications and Parameters

Biological Background: LXR beta/NER Function and Localization

• LXR beta (Oxysterols receptor LXR-beta, NR1H2) is a member of the nuclear receptor superfamily that functions as a ligand-activated transcription factor.
• Binds to DR-4 response elements (AGGTCA half-sites with 4-nt spacing) and regulates gene expression. Related references: PMID:25661920
• Regulates cholesterol uptake by promoting ubiquitination of LDLR, VLDLR, and LRP8 via MYLIP.
• In hepatocytes, induces LPCAT3-mediated phospholipid remodeling, which drives SREBF1 processing and de novo lipogenesis.
• Exhibits an anti-inflammatory role in the liver by acting as a corepressor to inhibit the hepatic acute phase response. Related references: PMID:20159957
• Predominantly localized to the nucleus and ubiquitously expressed across tissues.
• Contains zinc-finger DNA-binding domains, is subject to alternative splicing, and undergoes ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human LXR beta; consider verifying reactivity in target species due to high conservation.
• For Western Blot, the predicted molecular weight is ~51 kDa; use appropriate gel percentage and transfer conditions.
• For ELISA, the antibody can be used for direct or indirect detection; titrate antibody concentration for optimal signal-to-noise ratio.
• Include positive controls such as HepG2 or THP-1 cell lysates, or tissues with high LXR beta expression (e.g., liver).
• Since LXR beta is a nuclear receptor, nuclear extracts may enhance detection sensitivity.
• This antibody has been validated for Western Blot and ELISA; other applications may require further optimization.
• Note that alternative splicing variants exist; additional bands may be observed.

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CamelBio partners with diagnostic manufacturers and researchers to provide high-quality IVD raw materials like this LXR beta antibody. Our portfolio includes validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents for lipid metabolism and transcriptional regulation studies. We also offer customized sourcing for rare targets to accelerate your assay development.