Anti-Lutropin Subunit Beta (LHB) Polyclonal Antibody for WB, IHC-P, ELISA - P01229

Core Product Specifications and Parameters

Biological Background: Lutropin Subunit Beta (LHB) Function and Localization

• Encoded by the LHB gene, lutropin subunit beta is a component of luteinizing hormone (LH), a glycoprotein hormone secreted by the anterior pituitary gland.
• Combines with the common alpha subunit (CGA) to form the biologically active LH heterodimer, which acts on the gonads.
• LH promotes spermatogenesis in males and ovulation in females by stimulating the testes and ovaries to synthesize steroid hormones.
• The protein is secreted and contains structural features typical of the glycoprotein hormone family, including multiple disulfide bonds.
• Keywords associated with LHB include glycoprotein, hormone, and reference proteome; mutations are linked to hypogonadotropic hypogonadism.
• The predicted molecular weight of the mature LHB protein is approximately 15.3 kDa, consistent with the observed ~15 kDa band in Western blotting.
• Tissue specificity is restricted to the pituitary gland, where LH is produced and stored before release.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21-141 of human LHB, encompassing the mature secreted form; expect good reactivity with the processed protein in biological samples.
• For Western blotting of human pituitary extracts or cell lysates overexpressing LHB, a band near 15 kDa is anticipated.
• In immunohistochemistry (paraffin-embedded), antigen retrieval methods may be optimized for detection in pituitary tissue sections.
• ELISA protocols may benefit from checking cross-reactivity against related glycoprotein hormone beta subunits (e.g., CGB) if multiplexing.
• Validate antibody specificity in the relevant sample system, as endogenous expression is limited to the pituitary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for sourcing all IVD raw materials, from validated antibody pairs to optimized polyclonal antibodies like this anti-LHB pAb. We support reproductive hormone research and diagnostic assay development with bulk ancillary reagents and rare target sourcing. Let us help bridge your concept-to-clinic journey with comprehensive supply solutions.