Anti-Luteinizing Hormone Subunit Beta (LHB) Polyclonal Antibody for WB, IHC-P, ELISA - P01229

Core Product Specifications and Parameters

Biological Background: Lutropin Subunit Beta Function and Localization

• LHB encodes the beta subunit of luteinizing hormone (LH), a gonadotropin essential for reproductive function, including spermatogenesis and ovulation via steroid synthesis stimulation in testes and ovaries.
• The protein is also known as Lutropin subunit beta, CGB4, HH23, LSH-B, and LSH-beta, reflecting its role and historical nomenclature.
• LH is a heterodimeric glycoprotein hormone, with the beta subunit conferring specific biological activity and receptor binding, while the alpha subunit is shared with other glycoprotein hormones (FSH, TSH, hCG).
• Subcellular localization: Secreted; after processing, the mature hormone is released into the circulation to act on target gonadal tissues.
• Tissue specificity: Primarily expressed in the pituitary gland, consistent with its endocrine role in the hypothalamic-pituitary-gonadal axis.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for protein stability and activity; mutations in LHB are associated with hypogonadotropic hypogonadism.
• Molecular weight: approximately 15.3 kDa (precursor); the mature secreted form may undergo proteolytic processing.

Experimental Guidance and Technical Tips

• The immunogen spans a central region (aa 62-141) of human LHB; this region may be conserved across species, consistent with observed cross-reactivity with mouse and rat.
• For WB, the predicted molecular weight is ~15 kDa; validate using appropriate positive controls like pituitary lysates or recombinant protein.
• For IHC-P, optimize antigen retrieval and antibody dilution using pituitary tissue sections; endogenous peroxidases may require blocking.
• For ELISA, consider using the antibody as capture or detection; pairing with an anti-LH alpha subunit antibody or a matched antibody pair may enable sandwich ELISA development.
• As a polyclonal antibody, expect batch-to-batch variability; validate each lot in the intended application to ensure consistent performance.

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CamelBio supports diagnostic manufacturers and research laboratories with comprehensive sourcing solutions for IVD raw materials, including reliable polyclonal antibodies like this anti-LHB antibody for reproductive hormone research. In addition to antibodies, we provide validated antibody pairs, bulk ancillary reagents, and assistance in rare target raw-material procurement. Contact our team to streamline your supply chain from concept to clinic.