Anti-Lumican (LUM) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P51884

Core Product Specifications and Parameters

Biological Background: Lumican Function and Localization

• Lumican is a member of the small leucine-rich proteoglycan (SLRP) family, also known as keratan sulfate proteoglycan lumican (KSPG lumican).
• It is a secreted protein localized to the extracellular space and extracellular matrix, where it contributes to the organization and regulation of collagen fibrils.
• Lumican is predominantly expressed in the cornea and other connective tissues, playing a critical role in maintaining corneal transparency.
• The protein contains leucine-rich repeat (LRR) domains characteristic of SLRPs, which mediate interactions with collagen and other extracellular matrix components.
• Post-translational modifications include glycosylation (glycoprotein), phosphorylation (phosphoprotein), and sulfation, which can modulate its structural and functional properties.
• It features a pyrrolidone carboxylic acid modification at the N-terminus and contains disulfide bonds that stabilize its three-dimensional structure.
• The predicted molecular weight is 38,429 Da, with a detected band around 38 kDa in western blot applications.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 212–338 of human lumican, spanning part of the LRR domain; this region is likely conserved in mouse, supporting cross-reactivity.
• For western blot (WB), the expected band is ~38 kDa; use appropriate loading controls and consider the glycosylation status which may cause shifts.
• In immunohistochemistry (IHC-P) on paraffin-embedded sections, antigen retrieval conditions may need optimization; validate staining specificity in corneal tissue as a positive control.
• For ELISA applications, titrate the antibody against the recombinant protein or purified native lumican to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a comprehensive one-stop platform for IVD raw materials. This Lumican antibody supports studies in extracellular matrix biology and corneal transparency, and is part of our sourcing portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We also assist in procurement of rare target raw materials to accelerate your development pipeline.