Anti-LTF Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P02788

Core Product Specifications and Parameters

Biological Background: Lactotransferrin (LTF) Function and Localization

• Lactotransferrin (LTF) is a major iron-binding glycoprotein belonging to the transferrin family, which can bind two Fe³⁺ ions in association with bicarbonate (PubMed:11179314, PubMed:12693969, PubMed:14573629). Related references: PMID:11179314 PMID:12693969 PMID:14573629
• It is highly abundant in exocrine fluids such as breast milk, tears, and saliva, and plays a critical role in innate immunity through both iron sequestration and direct antimicrobial activity against bacteria, fungi, and biofilms (PubMed:6802759, PubMed:12037568, PubMed:11083624). Related references: PMID:6802759 PMID:12037568 PMID:11083624
• LTF modulates inflammatory responses by stimulating TLR4 signaling and NF-kB activation, while also interfering with LPS-stimulated TLR4 pathways, and inhibits neutrophil migration to apoptotic sites (PubMed:20345905, PubMed:19033648). Related references: PMID:20345905 PMID:19033648
• It promotes cell proliferation and differentiation, showing anabolic effects on osteoblasts, stimulation of VEGF-mediated endothelial cell migration, and anti-apoptotic activity (PubMed:15166119, PubMed:16842782). Related references: PMID:15166119 PMID:16842782
• LTF binds a variety of molecules including heparin, chondroitin sulfate, bacterial lipopolysaccharide, and DNA, which may contribute to its antimicrobial and immunomodulatory functions (PubMed:9359845). Related references: PMID:9359845
• The transferrin-like domain 1 exhibits serine protease activity (peptidase S60 family), cleaving arginine-rich sequences, which enhances its antimicrobial properties (PubMed:12535064). Related references: PMID:12535064
• A truncated isoform, DeltaLf, acts as a transcription factor, binding to promoter elements of genes like SKP1 and BAX to induce cell cycle arrest (PubMed:15222485, PubMed:22320386). Related references: PMID:15222485 PMID:22320386
• Tissue expression is broad: high in saliva/tears, moderate in serum/plasma, low in urine; detected in distal collecting tubules of kidney and peripheral blood neutrophils. Isoform DeltaLf is expressed in placenta, liver, ovary, while full-length LTF is abundant in kidney medulla, brain, and leukocytes.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein spanning amino acids 21–352 of human LTF. For Western blot, the predicted molecular weight is ~78 kDa; consider testing whole cell lysates from appropriate tissues (e.g., mammary, salivary) or secreted fractions given LTF’s exocrine nature, and validate in your sample system.
• For immunohistochemistry (IHC-P), appropriate fixation and antigen retrieval conditions should be empirically determined. LTF is highly expressed in breast, kidney medulla, and salivary glands, which can serve as positive tissue controls.
• In ELISA applications, the antibody can be used for detection of LTF in biological fluids like milk, tears, or serum; consider pairing with a matched detection system and standard curve.
• Due to its polyclonal nature, lot-to-lot variation may occur; inclusion of appropriate controls and validation within your specific experimental context is recommended.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and custom solutions. This anti-LTF polyclonal antibody supports research into iron metabolism, innate immunity, and diagnostic assay development. Whether you need optimized antibody pairs, tailored recombinant proteins, or rare target reagents, CamelBio streamlines your path from concept to clinic.