Anti-LTBR Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P36941

Core Product Specifications and Parameters

Biological Background: LTBR Function and Localization

• LTBR (TNFRSF3) is a member of the tumor necrosis factor receptor superfamily and is also known as lymphotoxin-beta receptor, TNFCR, TNFR3, TNFR-RP, TNFRSF3, TNFR2-RP, LT-BETA-R, and TNF-R-III.
• It functions as a receptor for heterotrimeric lymphotoxin (composed of LTA and LTB) and for TNFS14/LIGHT. Ligand binding activates the NF-kappa-B signaling pathway. Related references: PMID:24248355
• Through adaptor proteins TRAF3 and TRAF5, LTBR promotes apoptosis.
• It may play a role in the development of lymphoid organs.
• LTBR is localized to the plasma membrane.
• The protein undergoes post-translational modifications including disulfide bond formation, glycosylation, and phosphorylation.
• Alternative splicing of the LTBR gene results in multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular C-terminal region (aa 336-435) of human LTBR, ensuring specific detection of endogenous receptor.
• For western blot (WB), the predicted molecular weight is 47kDa; however, the protein may exhibit altered migration due to glycosylation.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), membrane staining is expected; appropriate antigen retrieval and controls should be optimized.
• Cross-reactivity with mouse and rat LTBR supports comparative studies; validate using species-appropriate lysates or tissues.
• The antibody is suitable for ELISA-based detection; titrate antibody concentration according to plate format and assay conditions.
• Perform standard blocking and antibody diluent optimization to reduce nonspecific binding in these applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a one-stop source for IVD raw materials, technical support, and consulting—streamlining development from concept to clinic. This anti-LTBR polyclonal antibody is offered alongside a broad portfolio of validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents for apoptosis, NF-κB signaling, and immunological studies. For rare targets or custom sourcing needs, CamelBio's expert teams facilitate reliable, scalable procurement to accelerate your assay development and production timelines.