Anti-LTBP3 Polyclonal Antibody for WB, ELISA - Q9NS15

Core Product Specifications and Parameters

Biological Background: LTBP3 Function and Localization

• Latent-transforming growth factor beta-binding protein 3 (LTBP3) is a member of the LTBP family, existing in multiple isoforms due to alternative splicing.
• LTBP3 acts as a key regulator of TGF-beta (TGFB1, TGFB2, TGFB3) activation by binding the latency-associated peptide (LAP) via disulfide bonds, maintaining TGF-beta in a latent state within the extracellular matrix.
• It regulates integrin-dependent activation of TGF-beta, enabling controlled release of the active growth factor.
• The protein is secreted and localizes primarily to the extracellular space and extracellular matrix.
• Isoform 2 is prominently expressed in heart, skeletal muscle, prostate, testis, small intestine, and ovary, while isoform 1 is strongly expressed in pancreas and liver. Related references: PMID:12154076
• LTBP3 contains EGF-like domains and is a glycoprotein, which may facilitate interactions with other matrix components.
• Genetic variants in LTBP3 have been associated with diseases such as amelogenesis imperfecta and dwarfism.

Experimental Guidance and Technical Tips

• Due to cross-reactivity with rat, consider validating the antibody in both human and rat sample systems for comparative studies.
• For Western blot, the predicted molecular weight is approximately 139 kDa; ensure the use of an appropriate gel percentage (e.g., 6–8% SDS-PAGE) and optimized transfer conditions for high-molecular-weight proteins.
• The immunogen corresponds to a C-terminal region (aa 1004–1303)—confirm that the detected band reflects the expected isoforms if multiple splice variants are present.
• In ELISA, this polyclonal antibody can be explored as a capture or detection reagent; empirical pairing and titer optimization are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting diagnostic manufacturers and research institutes from concept to clinic. For investigators studying TGF-beta signaling and extracellular matrix biology, this LTBP3 antibody serves as a reliable component for assay development. Explore our one-stop sourcing services to accelerate your transition from early research to clinical applications.