Anti-LTA4H Polyclonal Antibody for WB and ELISA - P09960

Core Product Specifications and Parameters

Biological Background: Leukotriene A-4 hydrolase Function and Localization

• Leukotriene A-4 hydrolase (LTA4H) is a bifunctional zinc metalloenzyme possessing both epoxide hydrolase and aminopeptidase activities (UniProt P09960).
• The epoxide hydrolase activity catalyzes the conversion of leukotriene A4 (LTA4) to the pro-inflammatory mediator leukotriene B4 (LTB4). Related references: PMID:11917124, PMID:12207002, PMID:15078870
• LTA4H can also use LTA5 as a substrate, albeit less efficiently, to produce LTB5. Related references: PMID:2996528
• The aminopeptidase activity displays high affinity for N-terminal arginines of tripeptides and inactivates the neutrophil chemoattractant Pro-Gly-Pro (PGP) by cleavage, thus counteracting inflammation. Related references: PMID:18804029, PMID:20813919, PMID:24591641
• LTA4H is involved in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, which are lipid mediators with potent anti-inflammatory and pro-resolving actions. Related references: PMID:21206090
• The enzyme localizes to the cytoplasm and is expressed across multiple blood cell types, including monocytes, lymphocytes, neutrophils, reticulocytes, platelets, and fibroblasts, in both isoform 1 and isoform 2.
• Key post-translational modifications include acetylation and phosphorylation, and the protein is a zinc-dependent metalloprotease.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 312–611 of human LTA4H (NP_000886.1), covering the C-terminal region. This region includes the catalytic domain and may recognize both the full-length protein and potential isoforms.
• For Western blot (WB), the predicted molecular weight is ~69 kDa. Consider using whole cell lysates from human or mouse leukocyte-rich samples (e.g., peripheral blood mononuclear cells) or cell lines such as THP-1 or RAW264.7, which are known to express LTA4H.
• For ELISA, the antibody can be used as a capture or detection reagent after in-house validation. Pair it with a matched monoclonal antibody if available for sandwich ELISA to detect soluble LTA4H in serum or plasma.
• Since cross-reactivity is listed for human and mouse, verify reactivity with your specific sample type and consider using blocking peptides to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-LTA4H polyclonal antibody, targeting a key enzyme in leukotriene signaling, complements our portfolio of reagents for inflammatory disease research. We offer bulk quantities, validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents, along with rare-target raw-material sourcing to streamline your assay development.