Anti-LTA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01374

Core Product Specifications and Parameters

Biological Background: LTA Function and Localization

• Lymphotoxin-alpha (LTA), also known as TNF-beta, is a cytokine and member of the tumor necrosis factor ligand superfamily (TNFSF1).
• In its homotrimeric form, LTA binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR, and TNFRSF14/HVEM, mediating inflammatory and immune responses.
  Related references: PMID:9462508
• As a heterotrimer with LTB, it engages TNFRSF3/LTBR, important for lymphoid organogenesis and immune cell homeostasis.
  Related references: PMID:24248355
• LTA is produced by lymphocytes and exhibits direct cytotoxicity against a broad range of tumor cells both in vitro and in vivo.
• The protein exists as a secreted and membrane-bound form, allowing both paracrine and juxtacrine signaling.
• It is post-translationally modified by glycosylation and contains a signal peptide.
• LTA has been characterized by 3D structure determination, direct protein sequencing, and proteomics identification, and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 35-205 of human LTA, covering the mature secreted form. This design ensures recognition of both native and recombinant protein. For WB, a band at ~22 kDa is expected; validate in relevant cell lines or tissues.
• For IHC-P, optimize antigen retrieval and antibody dilution using known positive control tissues such as lymph node or spleen.
• Cross-reactivity with mouse LTA is reported; consider this for cross-species studies.
• For ELISA, pair with a suitable capture antibody if sandwich ELISA is intended; validate against recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, including validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. Our expertise in cytokines and immune targets ensures seamless sourcing for diagnostic development. For your LTA research, rely on CamelBio as your one-stop bridge from concept to clinic.