Anti-LSS Rabbit Polyclonal Antibody for WB and ELISA - P48449

Core Product Specifications and Parameters

Biological Background: LSS Function and Localization

• Lanosterol synthase (LSS), also known as oxidosqualene--lanosterol cyclase (OSC) or hOSC, is encoded by the LSS gene and exists in multiple isoforms due to alternative splicing.

• Catalyzes the cyclization of (S)-2,3-oxidosqualene to lanosterol, forming the sterol nucleus, a key reaction in cholesterol biosynthesis (PubMed:14766201, PubMed:26200341, PubMed:7639730). Related references: PMID:14766201, PMID:26200341, PMID:7639730

• Through lanosterol production, may regulate lens protein aggregation and increase transparency, with implications for cataract formation (PubMed:26200341). Related references: PMID:26200341

• Localized to the endoplasmic reticulum membrane, reflecting its role in sterol biosynthesis.

• Widely expressed, with high levels in hair follicle epithelium (outer root sheath, matrix), dermal papilla, epidermis, sweat glands, sebaceous glands, and blood vessels.

• Post-translationally acetylated, and mutations are linked to congenital cataract (CTRCT44), hypotrichosis (HYPT14), and intellectual disability (APMR4).

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1–300, the N-terminal region of human LSS; evaluate sequence conservation with mouse for cross-reactivity.
• For Western blot, consider using HepG2 or HeLa cell lysates as positive controls; expect a band near 83 kDa.
• Validate the antibody in the intended assay system with appropriate species controls and optimize the working concentration.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supports your cholesterol metabolism research with high-quality reagents like this anti-LSS polyclonal antibody. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—streamlining your path from concept to clinic.