Anti-LSR Polyclonal Antibody for WB and ELISA - Q86X29

Core Product Specifications and Parameters

Biological Background: LSR Function and Localization

• LSR (Lipolysis-stimulated lipoprotein receptor), also known as Angulin-1, is a transmembrane receptor encoded by the LSR gene (alternative names: ILDR3, LISCH).
• It plays a probable role in the clearance of triglyceride-rich lipoproteins from the blood, binding chylomicrons, LDL, and VLDL in the presence of free fatty acids to facilitate cellular uptake (By similarity).
• LSR is essential for maintaining epithelial barrier function by recruiting MARVELD2/tricellulin to tricellular tight junctions (By similarity).
• The protein localizes to the cell membrane and specifically to tight junctions, indicating its role in cell-cell adhesion and barrier integrity.
• LSR undergoes alternative splicing and contains immunoglobulin domains, disulfide bonds, and is subject to post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin conjugation.
• Its molecular weight is approximately 71.4 kDa (UniProt calculated), consistent with the observed 71 kDa band.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider starting with a dilution range of 1:500-1:2000 and validate using lysates from human, mouse, or rat tissue/cell lines known to express LSR.
• In ELISA, titrate the antibody to determine the optimal coating concentration for indirect ELISA or as a capture/detection pair.
• The immunogen spans the N-terminal extracellular region (amino acids 50–240), which includes immunoglobulin-like domains; this region may be conserved across species, supporting the observed cross-reactivity.
• Since LSR is a transmembrane protein, ensure proper membrane extraction protocols for WB, and consider using reducing conditions to account for potential disulfide bonds.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for diagnostic manufacturers, providing validated antibody pairs, bulk ancillary reagents, and rare target raw materials. This anti-LSR polyclonal antibody supports research into lipoprotein clearance and epithelial barrier function, areas critical for metabolic and gastrointestinal disease diagnostics. Our team can assist with custom sourcing and supply chain solutions to accelerate your assay development from concept to clinic.