Anti-LSP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P33241

Core Product Specifications and Parameters

Biological Background: LSP1 Function and Localization

• Lymphocyte-specific protein 1 (LSP1) is a 47 kDa actin-binding protein also known as 52 kDa phosphoprotein (pp52) or lymphocyte-specific antigen WP34, encoded by the LSP1 gene.
• LSP1 may play a role in mediating neutrophil activation and chemotaxis, although its precise molecular mechanism remains partially defined.
• The protein undergoes alternative splicing, generating multiple isoforms that may contribute to functional diversity.
• Post-translational modifications such as phosphorylation and acetylation regulate LSP1 activity and interactions.
• LSP1 is predominantly localized to the cell membrane, consistent with its proposed role in signal transduction during cell activation and migration.
• Expression of LSP1 is mainly observed in activated T-lymphocytes, highlighting its specialized function in the immune system.
• The three-dimensional structure of LSP1 has been determined, revealing details of its actin-binding domain.
• Direct protein sequencing and proteomics studies have confirmed the identity and modification states of endogenous LSP1.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to full-length human LSP1 (amino acids 1-339), which can be expected to recognize the native protein across multiple applications.
• For Western Blot, the predicted molecular weight is approximately 37 kDa; optimize gel percentage and transfer conditions accordingly.
• In IHC-P, consider heat-induced epitope retrieval (HIER) in citrate buffer (pH 6.0) to improve antigen accessibility.
• ELISA protocols may require titration of both coating antigen and antibody concentrations for optimal signal.
• Cross-reactivity with mouse LSP1 suggests potential utility in murine models; validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to essential raw materials for immunoassay development. For LSP1-related research and assay construction, we offer polyclonal antibodies validated for WB, IHC-P, and ELISA, along with options for matched antibody pairs, recombinant proteins, and bulk ancillary reagents. Our global sourcing network also supports procurement of rare-target raw materials, helping you advance projects in immune cell biology with reliable supply.