Anti-LSM4 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9Y4Z0

Core Product Specifications and Parameters

Biological Background: LSM4 Function and Localization

• LSM4, also known as Glycine-rich protein (GRP), is a U6 snRNA-associated Sm-like protein that belongs to the LSm family of RNA-binding proteins.
• It is a core component of the heptameric LSM2-8 complex, which binds specifically to the 3′-terminal U-tract of U6 snRNA, a critical step in spliceosome assembly.
  Related references: PMID:10523320
• LSM4 participates in pre-mRNA splicing by functioning within the U4/U6-U5 tri-snRNP complex and later as part of the precatalytic spliceosome (B complex).
  Related references: PMID:28781166
• The protein localizes predominantly to the nucleus, consistent with its role in splicing.
• Post-translational modifications include acetylation and isopeptide bond formation; it is also targeted by ubiquitin conjugation pathways.
• The predicted molecular mass of LSM4 is approximately 15.35 kDa, aligning with the observed band at ~15 kDa in denaturing gels.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human LSM4 protein (amino acids 1–139), suggesting recognition of native and denatured epitopes; consider this for applications requiring native conformation (e.g., IP).
• For Western blot, a 15% SDS-PAGE gel and optimized transfer conditions for low-molecular-weight proteins are recommended; the expected band is around 15 kDa.
• Cross-reactivity with mouse and rat has been confirmed; validate the antibody in the specific species and tissue system of interest before large-scale experiments.
• When using for immunoprecipitation, titrate the antibody and include appropriate IgG controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of high-quality IVD raw materials, offering validated polyclonal antibodies like this anti-LSM4 reagent to support spliceosome research and diagnostic assay development. Our integrated supply capabilities extend to antibody pairs, bulk ancillary reagents, and custom sourcing of rare-target materials, providing a reliable bridge from concept to clinic for diagnostic manufacturers and laboratories worldwide.