Anti-LSM3 Rabbit Polyclonal Antibody for WB, ELISA - P62310

Core Product Specifications and Parameters

Biological Background: LSM3 Function and Localization

• LSM3 (also known as SMX4, USS2, YLR438C) is a member of the Sm-like (LSm) protein family and a core component of the spliceosome, playing critical roles in pre-mRNA splicing.
• It assembles into the heptameric LSm2-8 complex that directly recognizes and binds the 3'-terminal U-tract of U6 snRNA, a key step in spliceosome formation. Related references: PMID:10523320
• LSM3 participates in the formation of the U4/U6-U5 tri-snRNP complex and the precatalytic B complex spliceosome, essential for intron excision from pre-mRNA. Related references: PMID:28781166
• Subcellularly, LSM3 localizes predominantly to the nucleus, consistent with its role in nuclear mRNA processing.
• The protein undergoes post-translational modifications including acetylation, as evidenced by proteomics and direct protein sequencing studies.
• Structural studies have resolved the 3D conformation of LSm3 in complex with other LSm proteins, highlighting its RNA-binding and ribonucleoprotein structural roles.
• LSM3 is highly conserved across eukaryotes, with human LSM3 sharing significant sequence identity with mouse Lsm3, supporting strong cross-reactivity of this antibody between human and mouse samples.

Experimental Guidance and Technical Tips

• For Western blotting, consider using nuclear extract preparations from human or mouse cell lines to enrich target protein, as LSM3 is predominantly nuclear.
• Validate the antibody in your specific sample system, as detection may vary with expression levels or sample preparation methods.
• The immunogen covers almost the full-length LSM3 protein (aa 1-102), suggesting that the polyclonal antibody may recognize multiple epitopes; confirm specificity with appropriate controls (e.g., knockdown or overexpression lysates).
• For ELISA applications, optimize coating antigen (recombinant LSM3 protein) and antibody dilutions to achieve linear detection range.
• Store antibody as recommended (shipped on ice bag, long-term at -20°C) and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing validated antibody pairs, monoclonal and polyclonal antibodies, bulk reagents, and custom development services. This anti-LSM3 polyclonal antibody supports research into spliceosome biology and RNA processing, and CamelBio can supply complementary reagents such as secondary antibodies, ELISA kits, and related spliceosome targets. Our supply chain expertise ensures consistent lot-to-lot performance for diagnostic manufacturers and research labs.