Anti-LSM2 Polyclonal Antibody for WB, ELISA - Q9Y333

Core Product Specifications and Parameters

Biological Background: LSM2 Function and Localization

• LSM2, also known as protein G7b or snRNP core Sm-like protein Sm-x5, is a member of the Sm-like protein family involved in RNA processing.
• The heptameric LSM2-8 complex specifically binds to the 3'-terminal U-tract of U6 snRNA, a critical step in snRNP assembly.
  Related references:
  PMID:10523320
• LSM2 is essential for pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome (B complex).
  Related references:
  PMID:28781166
• The protein is localized to the nucleus, consistent with its role in mRNA processing.
• Post-translational modifications include phosphorylation, as indicated by its designation as a phosphoprotein.
• UniProt keywords classify LSM2 as a ribonucleoprotein with RNA-binding activity, underscoring its direct interaction with RNA.

Experimental Guidance and Technical Tips

• The polyclonal nature of this antibody may provide robust signal amplification; however, it is recommended to validate each new lot in your specific sample system to ensure consistent performance.
• The immunogen corresponds to amino acids 1-95 of human LSM2, targeting the N-terminal region. If your sample expresses isoforms lacking this domain, verify reactivity before use.
• For western blotting, the expected molecular weight is approximately 11 kDa. Use a high-percentage gel and optimize transfer conditions for small proteins to avoid loss.
• In ELISA, this antibody can be used for either capture or detection assays. Titrate the antibody and optimize coating conditions for best results.
• Include appropriate positive controls (e.g., nuclear extracts from human cell lines) and negative controls to facilitate interpretation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive one-stop platform for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-LSM2 antibody, bulk ancillary reagents, and rare target sourcing capabilities. Our expertise spans from early-stage research to clinical application, supporting your pre-mRNA splicing and spliceosome investigations with reliable supply chains and technical consultation.