Anti-LSM14A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8ND56

Core Product Specifications and Parameters

Biological Background: LSM14A Function and Localization

• LSM14A, also known as RAP55, FAM61A, or hRAP55A, is a conserved RNA-binding protein identified as a core component of processing bodies (P-bodies) and stress granules.
• It is essential for the formation of P-bodies, where it sequesters translationally inactive mRNAs and shields them from degradation, acting as a repressor of translation. Related references: PMID:16484376, PMID:17074753, PMID:29510985
• Beyond cytoplasmic mRNA processing, LSM14A contributes to mitotic spindle assembly, linking RNA metabolism with cell division. Related references: PMID:26339800
• Subcellularly, LSM14A localizes primarily to the cytoplasm, concentrating in P-bodies, stress granules, and the mitotic spindle apparatus.
• The protein undergoes multiple post-translational modifications such as acetylation, methylation, and phosphorylation, which likely modulate its function and subcellular distribution.
• Alternative splicing of the LSM14A gene produces several isoforms, potentially expanding its functional repertoire.
• Recognized as a developmental protein and a translation repressor, LSM14A may influence gene expression programs during differentiation and stress responses.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the expected band size is approximately 51 kDa; use whole-cell lysates from human, mouse, or rat lines known to express LSM14A (e.g., HeLa, HEK293, NIH/3T3) as positive controls.
• In immunofluorescence (IF/ICC), fixation and permeabilization protocols must be optimized to preserve P-body and stress granule structures; consider co-staining with markers like DCP1A or G3BP1 to confirm localization.
• For ELISA applications, the immunizing peptide (amino acids 1–100) can serve as a coating antigen to validate antibody specificity and titer.
• Since the antibody is raised against the N-terminal region, it may recognize multiple isoforms that share this domain; cross-validation with isoform-specific tools is recommended.
• The antibody cross-reacts with mouse and rat LSM14A, but sequence alignment is advisable for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with end-to-end access to high-quality IVD raw materials, coupled with expert technical and regulatory guidance. For researchers probing P-body biology, translational control, or RNA-protein interactions, we supply validated antibody pairs, recombinant proteins, and ancillary reagents ready for integration into commercial assays. Through our extensive global network, CamelBio sources even niche targets like LSM14A, streamlining your path from biomarker discovery to clinical application.