Anti-LSM12 Rabbit Polyclonal Antibody for WB, ELISA - Q3MHD2

Core Product Specifications and Parameters

Biological Background: LSM12 Function and Localization

• LSM12 is a nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein that confers NAADP sensitivity to the two-pore channel (TPC) complex, acting as an essential accessory protein for NAADP-evoked Ca2+ release from intracellular stores (PubMed:34362892). Related references: PMID:34362892
• The protein is localized to the cytoplasm, consistent with its role in modulating intracellular calcium signaling pathways.
• Alternative splicing generates multiple isoforms of LSM12, suggesting tissue- or context-specific regulation of its function.
• LSM12 is subject to post-translational modifications including acetylation and phosphorylation, which may modulate its activity or interactions.
• It is classified as a reference proteome component and has been identified through direct protein sequencing and proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N‑terminal region (amino acids 1–195) of human LSM12; this region may be present across all isoforms, but isoform-specific detection should be confirmed if required.
• For Western blotting, a band near 22 kDa is expected based on the predicted molecular weight; consider including appropriate positive controls (e.g., lysates from human or mouse cells) and validate band specificity in the relevant experimental system.
• This antibody may be suitable for ELISA applications; however, optimal dilution and detection conditions should be empirically determined using the target antigen.
• When studying NAADP‑dependent Ca2+ signaling, ensure that the antibody does not interfere with the NAADP binding site on LSM12 if functional assays are planned.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and researchers with one‑stop access to critical raw materials, including this anti‑LSM12 antibody for NAADP‑mediated calcium signaling research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare‑target raw materials to streamline your assay development and production workflows.