Anti-LSM11 Polyclonal Antibody for WB, ELISA - P83369

Core Product Specifications and Parameters

Biological Background: LSM11 Function and Localization

• LSM11, also known as U7 snRNA-associated Sm-like protein LSm11, is a core component of the U7 small nuclear ribonucleoprotein (U7 snRNP) complex.
• It plays a crucial role in histone mRNA 3'-end processing, enabling proper maturation of replication-dependent histone pre-mRNAs (PubMed:11574479, PubMed:16914750, PubMed:33230297). Related references: PMID:11574479, PMID:16914750, PMID:33230297
• Overexpression of LSM11 results in increased U7 snRNA levels but does not proportionally enhance histone pre-mRNA processing activity, indicating a regulatory rather than catalytic role (PubMed:11574479, PubMed:16914750). Related references: PMID:11574479, PMID:16914750
• LSM11 directly binds the Sm-binding site of U7 snRNA, a critical step for U7 snRNP assembly and function (PubMed:11574479, PubMed:16914750). Related references: PMID:11574479, PMID:16914750
• LSM11 is required for cell cycle progression from G1 to S phase, likely through its role in histone biogenesis (By similarity).
• The protein is localized exclusively to the nucleus, consistent with its function in pre-mRNA processing.
• Post-translational modifications, including methylation, phosphorylation, and ubiquitination, have been identified on LSM11, potentially modulating its stability or interactions.
• UniProtKB keywords link LSM11 to Aicardi-Goutieres syndrome (a type I interferonopathy), suggesting possible disease relevance, though direct functional evidence is limited.

Experimental Guidance and Technical Tips

• The immunogen (aa 131-360 of human LSM11) spans a significant portion of the protein, likely generating polyclonal antibodies that recognize multiple epitopes suitable for denaturing Western blotting.
• In Western blot, expect a band at approximately 40 kDa; use nuclear-enriched extracts or whole cell lysates from human or rat cell lines to enhance detection.
• For ELISA, the antibody may be used as a capture or detection reagent; titrate and validate with recombinant LSM11 protein or synthetic peptide.
• Given the nuclear localization, consider subcellular fractionation to improve Western blot signal-to-noise ratio.
• Cross-reactivity with rat is indicated; verify performance in your specific sample and application context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material sourcing partner for diagnostic manufacturers and research labs. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support research in RNA processing, cell cycle, and histone biology. This anti-LSM11 polyclonal antibody is part of our catalog, and our team can also source rare targets and provide technical consulting for your assay development needs.