Anti-LSM1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15116

Core Product Specifications and Parameters

Biological Background: LSM1 Function and Localization

• LSM1, also known as Cancer-associated Sm-like (CaSm) or CASM, is a U6 snRNA-associated Sm-like protein.
• Plays a critical role in the degradation of histone mRNAs, the only eukaryotic mRNAs that are not polyadenylated. Related references: PMID:18172165
• Functions as part of an LSm subunit-containing complex that participates in the general process of cytoplasmic mRNA decay (By similarity).
• Localizes primarily to the cytoplasm and is enriched in P-bodies, dynamic cytoplasmic foci involved in mRNA turnover.
• Belongs to the highly conserved Sm/LSm protein family, key components of ribonucleoprotein complexes involved in RNA processing.
• Phosphorylation has been reported, indicating potential regulatory mechanisms controlling its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–133) of human LSM1, which may be effective for detecting both native and denatured protein forms.
• For Western blotting, expect a band at approximately 15 kDa; validate in the relevant human or mouse cell/tissue lysate.
• In immunofluorescence/ICC, anticipate cytoplasmic staining with possible enrichment in P-body-like foci; co-staining with P-body markers (e.g., DCP1A) can confirm localization.
• For ELISA, optimize coating antigen concentration and antibody dilution for best signal-to-noise ratio.
• Since this is a polyclonal antibody, consistency across lots can vary; consider performing lot-specific validation for critical experiments.
• Cross-reactivity with mouse allows analysis in rodent models; verify performance in the specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. For research involving mRNA degradation and histone processing, CamelBio can supply the LSM1 Rabbit pAb along with complementary reagents and expert technical support to accelerate your diagnostic development or fundamental studies.