Anti-Lsh/HELLS Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - Q9NRZ9

Core Product Specifications and Parameters

Biological Background: Lsh/HELLS Function and Localization

• Lsh/HELLS is an ATP-dependent chromatin remodeler that facilitates de novo DNA methylation and recruits DNMTs/HDACs to promote transcriptional silencing, heterochromatin formation, and repression of transposable elements.
  Related references: PMID:30307408
• It is recruited to double-strand breaks in heterochromatin, aiding DNA repair via homologous recombination by mediating RBBP8/CtIP-dependent end resection.
  Related references: PMID:22946062 | PMID:31802118
• During meiosis, PRDM9 recruits HELLS to recombination hotspots to facilitate chromatin opening, enabling meiotic recombination.
  Related references: PMID:32001511
• It is involved in the regulation of expansion or survival of lymphoid cells (By similarity).
• HELLS localizes to the nucleus and associates with chromosomes.
• Highly expressed in proliferative tissues such as thymus and testis, and in neoplastic cell lines; lower expression in uterus, small intestine, colon, and peripheral blood mononuclear cells.
• Contains ATP-binding and helicase domains, is subject to phosphorylation, and is linked to ICF4 syndrome (immunodeficiency-centromeric instability-facial anomalies syndrome 4).

Experimental Guidance and Technical Tips

• The immunogen (amino acids 614-838 of human HELLS, NP_060533.2) may be conserved across human, mouse, and rat, consistent with reported cross-reactivity. Validate the antibody in your specific sample system and assay conditions.
• This rabbit monoclonal antibody is validated for WB, IF/ICC, IF-P, IHC-P, and ELISA. For WB, expect a band at ~97 kDa; use appropriate molecular weight markers and positive/negative controls.
• Given HELLS's roles in chromatin remodeling, DNA repair, and epigenetic regulation, this antibody may be useful for studies of heterochromatin dynamics and genome stability. For IF/IHC, optimize fixation and permeabilization protocols based on your sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop sourcing for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom procurement for rare targets. The Lsh/HELLS (Q9NRZ9) antibody supports chromatin biology and epigenetic research, and we offer technical consultation to assist with assay development from concept to clinical application.