Anti-LRWD1 Polyclonal Antibody for WB, ELISA - Q9UFC0

Core Product Specifications and Parameters

Biological Background: LRWD1 Function and Localization

• LRWD1 (leucine-rich repeat and WD repeat-containing protein 1), also known as CENP-33 or ORCA, is a testis-specific protein that acts as a chromatin regulator at centromeres, telomeres, and replication origins.
• It is required for the G1/S cell cycle transition by recruiting and stabilizing the origin recognition complex (ORC) onto chromatin, enabling pre-replication complex formation.
• LRWD1 recognizes a combination of DNA methylation and histone repressive marks, including H3K9me3, H3K27me3, and H4K20me3, to target heterochromatin regions.
• The protein localizes to multiple chromosome structures: the centromere (including the kinetochore), telomeres, and has cytoplasmic association with the centrosome and cytoskeleton.
• It contains leucine-rich repeats and WD repeats, motifs that mediate protein-protein interactions and are consistent with its role in chromatin and ORC binding.
• LRWD1 is essential for silencing of major satellite repeats, contributing to heterochromatin maintenance and genome stability.
• Expression is predominantly testis-specific, and it is drastically down-regulated in testis from patients with Sertoli cell-only syndrome, indicating a role in spermatogenesis.
• Post-translational modifications include phosphorylation and ubiquitination (UBL conjugation), as identified by proteomics, suggesting regulatory mechanisms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 250-350 of human LRWD1; consider verifying the epitope accessibility in native versus denatured protein contexts for your specific assay.
• For WB, use standard protocols and validate with appropriate positive (testis-derived) and negative controls, as LRWD1 expression is tissue-specific.
• ELISA may be suitable for peptide or recombinant protein detection; perform cross-reactivity testing against mouse and rat orthologs if multispecies studies are planned.
• Due to LRWD1's low abundance and tissue-restricted expression, consider enrichment strategies or sensitive detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering a comprehensive portfolio of high-quality antibodies, including this LRWD1 polyclonal antibody, ideal for DNA replication and chromatin biology research. Whether you need validated antibody pairs, bulk ancillary reagents, or rare target sourcing, our one-stop supply chain supports your development from concept to clinic. Contact us to discuss your specific diagnostic assay requirements.