Anti-LRSAM1 Polyclonal Antibody for WB and ELISA - Q6UWE0

Core Product Specifications and Parameters

Biological Background: LRSAM1 Function and Localization

• Encoded by the LRSAM1 gene, also known as TAL, this protein is a leucine-rich repeat and sterile alpha motif-containing E3 ubiquitin-protein ligase that plays dual roles in endosomal sorting and antibacterial autophagy.
• Mediates monoubiquitination of TSG101 at multiple sites, inactivating TSG101's ability to sort endocytic (e.g., EGF receptor) and exocytic (e.g., HIV-1 viral proteins) cargos. Related references: PMID:15256501
• Acts as a bacterial recognition protein that defends the cytoplasm from invasive pathogens by localizing to intracellular bacteria and generating bacteria-associated ubiquitin signals, which trigger xenophagy (autophagy-mediated degradation). Related references: PMID:23245322, PMID:25484098
• LRSAM1 localizes to the cytoplasm and is highly expressed in adult spinal cord motoneurons, as well as in fetal spinal cord and muscle tissue.
• Belongs to the leucine-rich repeat (LRR) and sterile alpha motif (SAM) domain-containing family, featuring a C-terminal RING-type zinc finger essential for its E3 ligase activity.
• Post-translational modifications include phosphorylation, and mutations in LRSAM1 are linked to Charcot-Marie-Tooth disease type 2P (CMT2P), a peripheral neuropathy.
• Annotated with keywords covering alternative splicing, disease variant, neurodegeneration, and protein transport, reflecting its multifaceted roles in cellular trafficking and neuron survival.
• The protein has a predicted molecular weight of approximately 84 kDa, consistent with its complex domain architecture.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-290) of human LRSAM1, which includes the leucine-rich repeats; this design often enhances specificity for native protein detection.
• For Western blot, expect a band near 84 kDa, though actual migration may vary depending on post-translational modifications; validate with appropriate positive controls (e.g., spinal cord or muscle lysates).
• The antibody cross-reacts with mouse and rat LRSAM1 based on sequence homology, but users should verify reactivity in their specific sample type and application.
• While ELISA is listed as a supported application, optimization of coating antigen and antibody dilution is recommended; a recombinant LRSAM1 protein may serve as a useful standard.
• Due to the polyclonal nature, lot-to-lot consistency should be confirmed by the end user, especially for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with reliable access to antibodies like LRSAM1 Rabbit pAb, targeting ubiquitin ligases implicated in neurodegeneration and autophagy. We offer a broad portfolio of validated antibody pairs, optimized polyclonals, and ancillary reagents, streamlining procurement for projects in rare disease areas such as Charcot-Marie-Tooth. Our sourcing expertise ensures consistent supply of high-quality raw materials to accelerate your assay development from concept to clinic.