Anti-LRRK2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q5S007

Core Product Specifications and Parameters

Biological Background: LRRK2 Function and Localization

• LRRK2 is a large multidomain serine/threonine-protein kinase that phosphorylates a broad range of substrates, playing critical roles in neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. Related references: PMID:17114044 PMID:20949042 PMID:21850687

• It acts as a key regulator of RAB GTPases, controlling their GTP/GDP exchange and protein interactions through phosphorylation of multiple RABs including RAB3A-D, RAB5A-C, RAB8A/B, RAB10, RAB12, RAB29, RAB35, and RAB43. Related references: PMID:23395371 PMID:26824392 PMID:28720718 PMID:29125462

• LRRK2 regulates primary ciliogenesis and sonic hedgehog (SHH) signaling in the brain via RAB8A and RAB10 phosphorylation, and together with RAB29, mediates retromer-dependent retrograde trafficking between lysosomes and the Golgi. Related references: PMID:23395371 PMID:29125462 PMID:30398148

• Beyond kinase activity, LRRK2 possesses GTPase function through its Roc domain, which reciprocally regulates kinase activity, and is recruited by RAB29 to overloaded lysosomes to promote lysosomal content release and suppress enlargement via EHBP1/EHBP1L1 effectors. Related references: PMID:18230735 PMID:26824392 PMID:28720718 PMID:30209220 PMID:38227290

• LRRK2 positively regulates autophagy through a calcium-dependent CaMKK/AMPK pathway, involving NAADP receptor activation, lysosomal pH increase, and calcium release. Related references: PMID:22012985

• It modulates innate immunity by enhancing RIPK2 activation downstream of NOD1/NOD2, and independently of kinase activity, inhibits proteasomal degradation of MAPT (tau), promoting its oligomerization and secretion. Related references: PMID:27830463 PMID:26014385

• LRRK2 localizes to multiple subcellular compartments including cytoplasmic vesicles, Golgi, ER, mitochondria, endosomes, lysosomes, and synaptic vesicles; it is widely expressed in brain, heart, liver, and other tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 750–910 of human LRRK2, a region N-terminal to the leucine-rich repeat domain; the resulting polyclonal antibody may recognize multiple epitopes and could be suitable for detecting various isoforms or cleavage products.
• For Western blot, consider the high molecular weight of LRRK2 (~286 kDa); use a low-percentage polyacrylamide gel (e.g., 6–8%) and optimize transfer conditions (e.g., extended wet transfer) to ensure efficient blotting.
• In immunohistochemistry (IHC-P), antigen retrieval methods (heat-induced or enzymatic) may enhance detection; validate the antibody in your specific tissue and fixation conditions.
• Immunofluorescence/immunocytochemistry (IF/ICC) may require permeabilization and careful fixation to preserve epitope accessibility; co-staining with organelle markers can help confirm localization patterns.
• ELISA: the antibody can be used as a capture or detection reagent; consider pairing with a validated LRRK2 antibody clone for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For LRRK2-related Parkinson’s disease and neurodegeneration research, we support procurement of high-quality detection antibodies like this LRRK2 pAb, along with matched antigen standards and technical consulting to accelerate assay development. Contact us to source rare-target raw materials and streamline your IVD pipeline from concept to clinic.