Anti-LRRK2 Rabbit Monoclonal Antibody for WB, ELISA - Q5S007

Core Product Specifications and Parameters

Biological Background: LRRK2 Function and Localization

• LRRK2 (Leucine-rich repeat serine/threonine-protein kinase 2), also known as Dardarin or PARK8, is a large multidomain protein possessing both serine/threonine kinase and GTPase activities; the GTPase function via the Roc domain regulates its kinase activity. Related references: PMID:18230735, PMID:26824392, PMID:28720718
• This kinase phosphorylates a broad array of substrates involved in critical cellular processes including neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. Related references: PMID:17114044, PMID:20949042, PMID:21850687
• LRRK2 is a master regulator of RAB GTPases; it phosphorylates multiple RAB family members (RAB3, RAB5, RAB8, RAB10, RAB12, RAB29, RAB35, RAB43), thereby controlling their GDP/GTP exchange and interactions with effectors. Related references: PMID:23395371, PMID:26824392, PMID:28720718
• LRRK2 positively regulates autophagy via a calcium-dependent mechanism that activates the CaMKK/AMPK signaling pathway, involving lysosomal calcium release. Related references: PMID:22012985
• In neurons, LRRK2 modulates process morphology, synaptic vesicle trafficking, and primary ciliogenesis; it phosphorylates RAB8A and RAB10 to promote sonic hedgehog (SHH) signaling in the brain. Related references: PMID:17114044, PMID:24687852, PMID:29125462, PMID:30398148
• Mutations in the LRRK2 gene are the most common genetic cause of familial Parkinson’s disease (PARK8) and also contribute to sporadic cases.
• Subcellularly, LRRK2 localizes to numerous membrane compartments including the Golgi apparatus, endoplasmic reticulum, endosomes, lysosomes, mitochondria, and synaptic vesicles; it is also found in the cytosol and associated with the cytoskeleton.
• LRRK2 is broadly expressed, with high levels in brain, heart, and liver; in the central nervous system, it is prominent in dopaminoceptive areas and cortical pyramidal neurons.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 100–340 of human LRRK2, an N-terminal region; consider epitope accessibility in native versus denaturing conditions when designing detection assays.
• For Western blotting, use low-percentage SDS-PAGE gels and extended transfer times suitable for high-molecular-weight proteins (~286 kDa).
• In ELISA applications, validate detection using recombinant LRRK2 protein or lysates from cell lines that express the target endogenously.
• Cross-reactivity is confirmed for human; for other species, assess sequence homology of the immunogen region and perform a pilot experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive, one-stop access to IVD raw materials, spanning from concept to clinic. This anti-LRRK2 monoclonal antibody supports critical investigations into Parkinson’s disease, autophagy, and neuronal signaling pathways. Beyond single reagents, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development and production workflows.