Anti-LRRK1 Rabbit Polyclonal Antibody for WB and ELISA - Q38SD2

Core Product Specifications and Parameters

Biological Background: LRRK1 Function and Localization

• LRRK1 (Leucine-rich repeat serine/threonine-protein kinase 1), also known as OSMD, RIPK6, or Roco1, is a multidomain protein kinase involved in the regulation of intracellular trafficking.
• It specifically phosphorylates RAB GTPases, including RAB7A, in a protein kinase C-dependent manner, mediating vesicular transport and endolysosomal dynamics.
  Related references: PMID:36040231, PMID:37558661, PMID:37857821
• The protein contains structural motifs such as ankyrin repeats, leucine-rich repeats, a kinase domain, and WD repeats, enabling scaffold and enzymatic functions.
• Subcellular localization is primarily cytoplasmic and at the cell membrane, consistent with its role in membrane-associated trafficking processes.
• Functional studies, including by similarity, indicate a role in the negative regulation of bone mass, acting through osteoclast maturation.
• LRRK1 is a metal-dependent enzyme, requiring magnesium or manganese as cofactors, and is regulated by phosphorylation.
• The human LRRK1 gene (Gene ID 79705) encodes a 225 kDa protein, with alternative splicing generating distinct isoforms.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein spanning amino acids 10–90 of the N-terminal region, may allow detection of multiple splice variants if this region is conserved; verify in relevant tissues.
• For Western blotting, use standard reducing conditions; the full-length protein has a predicted molecular weight of 225 kDa, and appropriate molecular weight markers should be included.
• In ELISA applications, optimize coating antigen concentrations and consider using a blocking step to reduce non-specific binding.
• Cross-reactivity is confirmed only for human; for other species, validation is recommended due to the lack of cross-reactivity data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For targets such as LRRK1, which are relevant to intracellular trafficking and osteoclast biology, we provide reliable supply channels to support your assay development and research programs. Partner with CamelBio to streamline your raw-material procurement from concept to clinic.