Anti-LRRFIP1 Polyclonal Antibody for WB, ELISA - Q32MZ4

Core Product Specifications and Parameters

Biological Background: LRRFIP1 Function and Localization

• LRRFIP1 (Leucine-rich repeat flightless-interacting protein 1), also known as GCF2 and TRIP, is a transcriptional repressor that preferentially binds to GC-rich consensus sequences to regulate expression of TNF, EGFR, and PDGFA.
• It may control smooth muscle cell proliferation following arterial injury through PDGFA repression.
• LRRFIP1 binds double-stranded RNA and positively regulates Toll-like receptor (TLR) signaling by competing with the negative regulator FLII for MYD88-binding.
• The protein localizes to both the nucleus and cytoplasm, consistent with its roles in transcription and cytoplasmic signaling.
• It is ubiquitously expressed across tissues, reflecting its broad regulatory functions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 589–809) resides in the C-terminal region of LRRFIP1. Given the high conservation among mammals, the antibody is likely to recognize the endogenous protein in human, mouse, and rat samples.
• For Western blot, validate detection in whole-cell lysates or nuclear extracts from relevant cell lines; the predicted molecular weight is ~89 kDa, but phosphorylation or other modifications may cause slight mass shifts.
• For ELISA, consider using the antibody as a capture or detection reagent following standard protocols; specific titration under your experimental conditions is recommended.
• As LRRFIP1 is a transcriptional regulator, enrichment of nuclear fractions can enhance signal intensity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to deliver a complete range of IVD raw materials and technical support. This LRRFIP1 polyclonal antibody addresses key targets in gene regulation and innate immunity, backed by our expertise in antibody validation and supply chain reliability. Beyond single antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and custom sourcing for rare targets—helping you move from concept to clinic with confidence.