Anti-LRRC7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96NW7

Core Product Specifications and Parameters

Biological Background: LRRC7 Function and Localization

• Adapter protein LRRC7 is essential for correct postsynaptic architecture and modulates postsynaptic plasticity via multiple protein-protein interactions. Related references: PMID:11729199, PMID:39256359
• Through its association with CACNA1C, LRRC7 promotes trafficking and clustering of L-type voltage-gated calcium channels at dendritic membranes, enhancing excitatory calcium signaling.
• LRRC7 acts as a molecular scaffold that brings CAMK2A and PPP1CA into close proximity, likely facilitating rapid dephosphorylation of CAMK2A and influencing postsynaptic plasticity. Related references: PMID:39256359
• LRRC7 is required for the localization of DISC1 and GRM5 to postsynaptic density complexes and is involved in both NMDAR- and mGluR-dependent long-term synaptic depression.
• Subcellularly, LRRC7 is localized to the cytoplasm, specifically concentrated at the postsynaptic density of dendritic spines.
• The protein is predominantly brain-specific; however, isoform 3 exhibits ubiquitous expression across tissues.
• LRRC7 undergoes alternative splicing and is subject to post-translational modifications including phosphorylation and methylation. Genetic variants are linked to autism spectrum disorder and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 730-890 of human LRRC7, a region that may recognize multiple isoforms due to alternative splicing. When performing WB, consider using a positive control from brain lysate (human, mouse, or rat) and note the expected band around 173 kDa.
• For IF/ICC, validate the antibody in appropriate neuronal or transfected cell models, as LRRC7 localizes to dendritic spines and postsynaptic densities.
• This antibody cross-reacts with mouse and rat; ensure species-specific validation if used in non-human samples. Optimize dilution factors empirically for each application and model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research labs, and institutions a reliable one-stop channel for IVD raw materials, technical services, and consulting. This LRRC7 antibody addresses neuroscience and synaptic biology research demands, and we can supply validated antibody pairs, bulk ancillary reagents, and assist with sourcing rare-target raw materials for your workflow development. Partner with CamelBio to efficiently bridge the gap from concept to clinic.