Anti-LRRC4 Polyclonal Antibody for WB, ELISA - Q9HBW1

Core Product Specifications and Parameters

Biological Background: LRRC4 Function and Localization

• LRRC4 (Leucine-rich repeat-containing protein 4) is a synaptic adhesion protein that regulates the formation of excitatory synapses by recruiting pre- and postsynaptic proteins.
• It plays an important role in auditory synaptic responses and organizes the lamina/pathway-specific differentiation of dendrites.
• The protein is involved in the suppression of glioma, consistent with its alternative names Brain tumor-associated protein BAG and Nasopharyngeal carcinoma-associated gene 14 protein.
• LRRC4 is specifically expressed in brain tissue.
• Subcellular localization is at the cell membrane, specifically the postsynaptic cell membrane.
• Structural features include leucine-rich repeats, an immunoglobulin domain, a signal peptide, disulfide bonds, a transmembrane helix, and glycosylation sites (glycoprotein).

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to amino acids 544-653 of human LRRC4 (NP_071426.1). Ensure the target region is present in your protein of interest when using across species.
• For WB, consider standard loading controls and optimize antibody dilution based on protein abundance in your sample; brain tissue lysates are recommended given the specific expression pattern.
• In ELISA, the antibody can be used for detection of recombinant or native LRRC4; validate coating conditions and specificity using appropriate controls.
• Cross-reactivity with mouse and rat allows the antibody to be applied in rodent models; however, verify sequence homology across species for the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive source for IVD raw materials, supporting diagnostic manufacturers and research laboratories from early-stage development to clinical application. This polyclonal antibody targeting LRRC4 meets needs in neurobiology and oncology research, complementing our offerings of validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. For your project requiring reliable detection tools or raw material supply, connect with CamelBio to streamline procurement.