Anti-LRRC14 Rabbit Polyclonal Antibody for WB, ELISA - Q15048

Core Product Specifications and Parameters

Biological Background: LRRC14 Function and Localization

• LRRC14, also known as LRRC14A, is a leucine-rich repeat-containing protein encoded by the LRRC14 gene (Gene ID: 9684) in humans.
• It functions as a negative regulator of Toll-like receptor (TLR)-mediated NF-kappa-B signaling by disrupting IKK core complex formation via interaction with IKBKB (inhibitor of nuclear factor kappa-B kinase subunit beta).
• LRRC14 localizes to the cytoplasm where it exerts its regulatory role on innate immune signaling.
• The protein belongs to the leucine-rich repeat (LRR) superfamily, characterized by tandem LRR motifs typically involved in protein-protein interactions.
• UniProt annotates LRRC14 with keywords including cytoplasm, leucine-rich repeat, and reference proteome, highlighting its functional and structural domains.
• The predicted molecular weight of LRRC14 is approximately 55 kDa (UniProt: 54513 Da), consistent with immunoblot detection.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length LRRC14 protein (aa 1-280), suggesting the antibody may recognize multiple epitopes; consider using reduced/denatured conditions for Western blot to ensure linear epitope exposure.
• For Western blot (WB), a band at ~55 kDa is expected in human cell or tissue lysates; include appropriate positive and negative controls.
• For ELISA, the antibody can be used as a capture or detection reagent; validate coating concentrations and blocking conditions in your specific assay setup.
• Since LRRC14 is a cytoplasmic protein, consider subcellular fractionation to enrich the target for improved sensitivity.
• Cross-reactivity is confirmed for human; if using other species, verify sequence homology and test reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-LRRC14 polyclonal antibody is ideal for researchers investigating TLR-mediated NF-kappa-B signaling and innate immunity. Our comprehensive portfolio includes validated antibody pairs, bulk ancillary reagents, and rare target sourcing to support your immunoassay development and production needs.