Anti-LRPAP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30533

Core Product Specifications and Parameters

Biological Background: LRPAP1 Function and Localization

• LRPAP1 (Low density lipoprotein receptor-related protein-associated protein 1) is also known as RAP, alpha-2-MRAP, or HBP44, encoded by the LRPAP1 gene.
• It functions as a molecular chaperone for LDL receptor-related proteins, regulating their ligand binding activity along the secretory pathway.
• The protein localizes to the rough endoplasmic reticulum lumen, ER-Golgi intermediate compartment, Golgi apparatus (cis-Golgi network and lumen), endosome lumen, and cell surface.
• LRPAP1 is a glycoprotein and phosphoprotein, and it exhibits heparin-binding activity.
• Structural features include a coiled coil domain; the protein has been characterized by direct protein sequencing and 3D-structure studies.
• It is identified in proteomics analyses and serves as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 35-357 of human LRPAP1, excluding the signal peptide but containing the core functional regions; this sequence is conserved across human, mouse, and rat, explaining the observed cross-reactivity.
• For Western blot (WB), consider using reducing conditions and loading 20-40 µg of total protein lysate; the predicted molecular weight is 41 kDa, but glycosylation may alter migration.
• For immunohistochemistry (IHC-P), test antigen retrieval with citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) and optimize primary antibody dilution.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilization with 0.1-0.3% Triton X-100 or saponin and fixation with 4% paraformaldehyde are common starting points.
• For ELISA, validate the antibody as a capture or detection reagent in the relevant sample matrix; coating concentrations and blocking conditions require empirical optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing platform for IVD raw materials, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents to diagnostic manufacturers, laboratories, and research institutes. Our catalog includes validated tools like this anti-LRPAP1 polyclonal antibody, supporting investigations into lipid metabolism, receptor trafficking, and related diseases. With capabilities spanning bulk antibody supply, customized rare-target sourcing, and technical consultation from concept to clinic, CamelBio accelerates your assay development and production workflows.