Anti-LRP6 Rabbit Polyclonal Antibody for WB, ELISA - O75581

Core Product Specifications and Parameters

Biological Background: LRP6 Function and Localization

• LRP6 functions as a coreceptor in the canonical Wnt-Fzd-LRP5-LRP6 signaling complex, essential for beta-catenin stabilization and transcriptional activation. Related references: PMID:11357136 PMID:11448771 PMID:15778503

• Wnt-induced Fzd/LRP6 complex recruits DVL1 and AXIN1/GSK3B to the plasma membrane, forming signalosomes that prevent beta-catenin phosphorylation and degradation. Related references: PMID:16513652

• LRP6 is required for posterior patterning of the epiblast during gastrulation.

• Localized to the cell membrane, endoplasmic reticulum, and membrane rafts, reflecting its role in signal transduction and trafficking.

• Widely coexpressed with LRP5 during embryogenesis and in adult tissues, indicating overlapping functional roles.

• Post-translationally modified by phosphorylation, palmitoylation, and ubiquitination, which regulate its activity and stability.

• Disease-associated variants in LRP6 are linked to autosomal dominant coronary artery disease 2 (ADCAD2) and osteopetrosis.

Experimental Guidance and Technical Tips

• For Western blot detection of the 180 kDa LRP6 protein, consider using low-percentage SDS-PAGE gels and extended transfer conditions with PVDF membranes to ensure efficient transfer.
• Validate antibody specificity in your sample system using positive controls (e.g., LRP6‑overexpressing cells) and negative controls (e.g., siRNA‑mediated knockdown or CRISPR‑knockout samples).
• Since the immunogen targets the extracellular domain (amino acids 200‑300), confirm detection of full‑length versus potential shed or cleaved forms by comparing observed and predicted molecular weights.
• For ELISA, the immunizing peptide can serve as a positive control to optimize coating, blocking, and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner, supplying diagnostic developers with high‑quality antibodies and ancillary reagents from concept through clinical application. For researchers exploring Wnt signaling and related diseases, CamelBio can source this anti‑LRP6 polyclonal antibody together with complementary LRP5 antibodies, recombinant Wnt proteins, and labeled secondary detection reagents. With expertise in rare‑target sourcing and bulk customization, CamelBio accelerates your assay development and scale‑up needs.