Anti-LRP6 Rabbit Polyclonal Antibody for IF/ICC, ELISA - O75581

Core Product Specifications and Parameters

Biological Background: LRP6 Function and Localization

• LRP6 (Low-density lipoprotein receptor-related protein 6), also known as ADCAD2 or STHAG7, is a cell-surface coreceptor of the canonical Wnt/β-catenin signaling pathway. It forms the Wnt-Fzd-LRP5-LRP6 complex that triggers β-catenin signaling by inducing the aggregation of receptor-ligand complexes into ribosome-sized signalosomes (PubMed:11357136, PubMed:11448771, PubMed:15778503). Related references: PMID:11357136 PMID:11448771 PMID:15778503
• Upon Wnt stimulation, LRP6 recruits DVL1 polymers and the AXIN1/GSK3B destruction complex to the plasma membrane. This inhibits AXIN1/GSK3-mediated phosphorylation and proteasomal degradation of β-catenin, thereby stabilizing it for nuclear translocation and target gene activation (PubMed:16513652). Related references: PMID:16513652
• LRP6 is ubiquitously expressed, with widespread coexpression alongside LRP5 during embryogenesis and in adult tissues, underscoring its fundamental role in development and tissue homeostasis.
• Subcellularly, LRP6 localizes to the cell membrane, endoplasmic reticulum, and membrane rafts, consistent with its function as a transmembrane signaling receptor and its trafficking through the secretory pathway.
• Post-translational modifications of LRP6 include phosphorylation, palmitoylation, ubiquitination, and disulfide bond formation, which collectively regulate its signal transduction, endocytosis, and stability.
• As a key developmental protein, LRP6 is implicated in retinal angiogenesis, bone formation, and epiblast patterning during gastrulation (By similarity).

Experimental Guidance and Technical Tips

• The immunogen (aa 20-150) resides in the extracellular domain of human LRP6, which is exposed on the cell surface. This suggests suitability for immunofluorescence/immunocytochemistry (IF/ICC) to detect membrane-localized LRP6 in fixed and permeabilized cells.
• For ELISA assays, consider using the recombinant protein immunogen (corresponding to aa 20-150) as a positive control to establish standard curves and confirm antibody binding.
• Given its wide tissue distribution and transmembrane nature, validate the antibody in your specific cell line or tissue sample under optimized conditions, potentially including enrichment of membrane fractions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our LRP6 rabbit polyclonal antibody is part of a robust portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for Wnt signaling research. Whether you need assay development support or reliable supply chain solutions, CamelBio is your strategic partner in IVD development.