Anti-LRP5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75197

Core Product Specifications and Parameters

Biological Background: LRP5 Function and Localization

• Serves as a coreceptor with frizzled family receptors for Wnt proteins, transducing signals to activate the canonical Wnt pathway. Related references: PMID:11336703, PMID:11448771, PMID:11719191
• Plays a critical role in osteoblast proliferation and differentiation, thereby regulating bone mass. Related references: PMID:11719191
• Upon Wnt ligand binding, the Frizzled-LRP5 complex recruits AXIN1, leading to stabilization of beta-catenin and activation of TCF/LEF transcription factors. Related references: PMID:11336703, PMID:14731402, PMID:24706814
• Also acts as a coreceptor for norrin/NDP in association with FZD4, essential for beta-catenin-dependent signaling in retinal vascular development. Related references: PMID:16252235, PMID:27228167
• Localizes to the plasma membrane and the endoplasmic reticulum.
• Widely expressed in tissues, with highest levels observed in liver and aorta.
• Predicted to undergo glycosylation and phosphorylation; belongs to the LDL receptor family with multiple EGF-like domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1400–1500; verify sequence conservation in your species of interest if not testing human, mouse, or rat.
• For Western blotting, expect a band at ~179 kDa; use a low-percentage (e.g., 6–8%) SDS-PAGE gel for optimal high-molecular-weight protein separation.
• In immunofluorescence/ICC, include permeabilization to detect intracellular pools (endoplasmic reticulum) alongside membrane staining.
• Cross-reactivity with mouse and rat is reported; however, validate the antibody in your specific sample type and protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents spanning the entire development pipeline. For Wnt signaling, bone biology, or retinal vasculature studies, our LRP5 rabbit polyclonal antibody provides a reliable detection tool, with supply volumes tailored to your needs. Reach out to discuss custom sourcing and technical guidance that accelerates your assay development from concept to clinic.