Anti-LRP5 Rabbit Monoclonal Antibody for WB, ELISA - O75197

Core Product Specifications and Parameters

Biological Background: LRP5 Function and Localization

• LRP5, also known as LRP-7, is a single-pass type I membrane protein and a member of the low-density lipoprotein receptor family.
• LRP5 acts as a coreceptor with Frizzled family proteins to transduce Wnt signals and activate the canonical Wnt/β-catenin pathway, regulating cell fate determination, self-renewal, and embryonic development. Related references: PMID:11336703, PMID:11448771, PMID:11719191
• During bone development, LRP5 regulates osteoblast proliferation and differentiation, thereby determining bone mass. Related references: PMID:11719191
• Mechanistically, the formation of the Wnt-Frizzled-LRP5 signaling complex promotes AXIN1 recruitment, stabilizing β-catenin and activating TCF/LEF transcriptional programs. Related references: PMID:11336703, PMID:14731402, PMID:24706814
• LRP5 also acts as a coreceptor for non-Wnt proteins such as Norrin/NDP, triggering β-catenin-dependent signaling essential for retinal vascular development. Related references: PMID:16252235, PMID:27228167
• Subcellularly, LRP5 localizes to the cell membrane and endoplasmic reticulum.
• Widely expressed with highest levels in liver and aorta; associated with diseases including osteoporosis, osteopetrosis, and retinopathies.
• Post-translationally modified by glycosylation and phosphorylation; contains EGF-like domains and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1400–1500) of human LRP5; consider validating reactivity with full-length protein or relevant cell lysates.
• Validated for Western blot (WB) and ELISA. Cross-reacts with human and mouse LRP5 (predicted MW ~179kDa); include appropriate positive/negative controls.
• Suitable for studying Wnt signaling, osteoblast differentiation, retinal vascular development, and associated pathologies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, supporting every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, including rare-target raw materials like Wnt signaling components. With this anti-LRP5 rabbit monoclonal antibody, CamelBio empowers diagnostic and research applications in bone disorders, vascular biology, and oncology.