Anti-LRP4 Rabbit Polyclonal Antibody for IF-P, ELISA - O75096

Core Product Specifications and Parameters

Biological Background: LRP4 Function and Localization

• LRP4 (Low-density lipoprotein receptor-related protein 4) is a transmembrane receptor encoded by the LRP4 gene. It is also known as MEGF7 (Multiple epidermal growth factor-like domains 7), LRP10, and SOST2, and belongs to the LDL receptor family.
• LRP4 mediates SOST-dependent inhibition of bone formation by functioning as a specific facilitator of SOST-mediated inhibition of Wnt signaling.
• It plays a crucial role in the formation and maintenance of the neuromuscular junction (NMJ) by directly binding AGRIN and recruiting it to the MUSK signaling complex, which induces MUSK phosphorylation and subsequent clustering of acetylcholine receptors (AChR) in the postsynaptic membrane.
• LRP4 may also negatively regulate the canonical Wnt signaling pathway by antagonizing LRP6-mediated activation.
• It has been proposed to function as a cell surface endocytic receptor that binds and internalizes extracellular ligands for degradation by lysosomes.
• (Microbial infection) LRP4 acts as a receptor for yellow fever virus.
• Subcellular location: Predominantly localized to the cell membrane.
• Tissue specificity: Expressed in bone (osteoblasts and osteocytes) and in several regions of the brain. Not expressed in osteoclasts.
• Key structural features include EGF-like domains, calcium-binding sites, disulfide bonds, and glycosylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• Since this is a polyclonal antibody generated against a synthetic peptide spanning amino acids 1621–1720 of human LRP4, it may recognize multiple epitopes and exhibit reactivity to post-translational modifications or isoforms. Validate specificity in your experimental system.
• For immunofluorescence on paraffin-embedded sections (IF-P), antigen retrieval methods may be necessary to expose the epitope. Optimize fixation and permeabilization conditions based on the tissue type.
• For ELISA applications, consider using this antibody as a detection or capture reagent, and include appropriate standard curves and controls to ensure assay linearity and specificity.
• The predicted molecular weight of LRP4 is 212 kDa; if performing Western blot (not validated by the supplier), ensure adequate gel electrophoresis and transfer conditions for high-molecular-weight proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined procurement pathway for IVD raw materials and technical support across the development pipeline. For LRP4-related research—including bone metabolism disorders, neuromuscular diseases, and viral entry studies—we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions. Partner with us to accelerate your assay development and scale-up with confidence.