Anti-LRP2 (Megalin) Rabbit Polyclonal Antibody for WB, IHC-P, IF-P - P98164

Core Product Specifications and Parameters

Biological Background: LRP2 Function and Localization

• LRP2 encodes megalin (gp330), a ~522 kDa type I transmembrane glycoprotein belonging to the LDL receptor family, functioning as a multiligand endocytic receptor expressed primarily at apical surfaces of absorptive epithelia.
• In the kidney, megalin mediates tubular uptake and clearance of diverse substrates including metallothionein-bound heavy metals, antiapoptotic survivin, 25-hydroxyvitamin D3, vitamin D3 transporter GC/DBP, and myoglobin, contributing to renal reabsorption and homeostasis. Related references: PMID:15126248 PMID:23825075
• Forms an endocytic complex with cubilin (CUBN) to mediate reuptake of filtered proteins such as albumin, vitamin carriers, and lipoproteins, preventing urinary loss.
• In the central nervous system, megalin regulates neurogenesis and brain development by mediating endocytosis of sonic hedgehog (SHH), leptin, and BMP4, and facilitates transcytosis of SHH and leptin across the blood-brain barrier.
• Subcellularly, LRP2 localizes to coated pits, apical cell membranes, endosome lumens, and neuronal projections (axons and dendrites), where it orchestrates clathrin-mediated internalization and trafficking.
• Tissue distribution is highest in renal proximal tubules, placental cytotrophoblasts, and choroid plexus epithelium, with roles in embryonic development, vitamin/ion homeostasis, and hearing. Related references: PMID:27798286
• Post-translational modifications include extensive glycosylation, disulfide bonds in EGF-like repeats, and phosphorylation, which regulate ligand binding and receptor recycling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal cytoplasmic domain (aa 4446‑4655), which is highly conserved across species, likely contributing to cross‑reactivity with mouse and rat. Verify sequence homology in your target species.
• For Western blot, use a low‑percentage gel (e.g., 6% SDS‑PAGE) and extended transfer times to resolve the 522 kDa full‑length protein. Validate band specificity using positive control lysates from kidney or brain tissue.
• Due to heavy glycosylation, the observed molecular weight may shift upward; consider enzymatic deglycosylation if precise sizing is required.
• In IHC‑P and IF‑P, optimize antigen retrieval and include a positive control such as human kidney sections. Signal may be membrane‑associated or punctate in endocytic vesicles.
• For ELISA, the polyclonal nature provides high avidity; validate with recombinant LRP2 or cell lysates to confirm sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing for LRP2 and related research areas, offering validated polyclonal antibodies, antigen‑grade proteins, and ancillary reagents. Whether you need bulk quantities, custom antibody development, or rare‑target raw materials for endocytosis, renal biology, or neurodevelopment studies, our integrated supply chain and technical consulting help bridge from concept to clinic. Contact us for one‑stop access to reliable, high‑quality reagents tailored to your diagnostic or research workflow.