Anti-LRIG1 Rabbit Polyclonal Antibody for WB and ELISA - Q96JA1

Core Product Specifications and Parameters

Biological Background: LRIG1 Function and Localization

• LRIG1 is a single-pass transmembrane protein containing leucine-rich repeats (LRR) and immunoglobulin-like (Ig) domains.
• It functions as a feedback negative regulator of receptor tyrosine kinase (RTK) signaling, promoting receptor ubiquitination and lysosomal degradation.
• LRIG1 is localized at the cell membrane, with its extracellular region mediating interactions with RTKs.
• The protein is widely expressed in human tissues, indicating a broad regulatory role.
• Alternative splicing produces multiple transcript variants encoding different isoforms.
• Structurally, LRIG1 includes an N-terminal signal peptide, LRR domains, three Ig-like domains, a transmembrane helix, and a cytoplasmic tail.
• Key post-translational modifications include glycosylation and disulfide bond formation, typical of cell surface receptors.

Experimental Guidance and Technical Tips

• The antibody was raised against a C-terminal fragment (aa 816-1093) of human LRIG1, targeting the cytoplasmic region. For western blot, validate the band specificity using appropriate controls (e.g., knockdown or knockout samples).
• A specific band at approximately 119 kDa corresponding to full-length LRIG1 may be observed. Consider using reducing SDS-PAGE and standard blocking conditions.
• For ELISA, optimize the coating and detection antibody concentrations based on the assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents for diagnostic development. Our LRIG1-related portfolio supports research in receptor tyrosine kinase signaling and cancer diagnostics. For bulk orders or customized sourcing of antibodies against rare targets, contact our B2B team.